Figures & data
Figure 1. Procedure of the in vivo study. BUN, blood urea nitrogen; 8-OHdG, 8-hydroxy-2’-deoxyguanosine; IHC, immunohistochemical.
Figure 2. Track map of Morris water maze on day 6. a and b, the track map of Morris water maze in sham-operated group; c and d, the track map of Morris water maze in 5/6 nephrectomy group.
Figure 3. Escape latency of Morris water maze in sham-operated group and 5/6 nephrectomy group. a, training day 1-5. Specific data were shown in Supplementary Table S3. *: p < 0.05. b, experimental day 6. *: p < 0.05.
Table 1. Hippocampal neuron count in two groups.
Figure 4. 8-OHdG immunohistochemistry staining of hippocampus in two groups. a, sham-operated group (200×); b, 5/6 nephrectomy group (200×); c, sham-operated group (400×); d, 5/6 nephrectomy group (400×). CA1-CA4, the CA1-CA4 region of hippocampal gyrus; DG, dentate gyrus.
Table 2. Cell counts and IOD values of 8-OHdG IHC staining in two groups.
Figure 5. Correlation analysis on time in quadrant IV. r, correlation coefficient; p, significance of correlation analysis. *: p < 0.05.
Figure 6. Cell viability and oxidative stress level of LPS-stimulated microglial cells. a, relative cell viability of LPS-stimulated microglial cells pretreated with either benfotiamine or folic acid. ***: p < 0.001. b, 8-OHdG concentration in culture supernatants of LPS-stimulated microglial cells pretreated with either benfotiamine or folic acid. Specific data were shown in Supplementary Table S4. *: p < 0.05.
