The effect of probiotic drinks containing homofermentative or hetrofermentative strains of lactobacilli on the growth of Candida albicans and Escherichia coli in mixed culture

Figures & data

Table I.  Actimel (L. casei Immunitas®) versus C. albicans and E. coli in mixed culture grown statically at 37°C.

Time (h)	Viable Actimel cell count (cfu/ml)	Viable C. albicans cell count (cfu/ml)
0	7×10^6	4×10^5
2	2×10^7	7×10^5
4	6.67×10^7	1.83×10^6
6	1.5×10^8	3×10^6
24	2×10^9	5×10^3
Time (h)	Viable Actimel cell count (cfu/ml)	Viable E. coli cell count (cfu/ml)
0	2×10^7	7.33×10^6
2	6.5×10^7	1.03×10^7
4	3×10^8	1.67×10^7
6	6×10^8	1.5×10^7
24	4.5×10^8	0

Table II.  Orchard Maid (L. reuteri) versus C. albicans and E. coli in mixed culture grown statically at 37°C.

Time (h)	Viable Orchard Maid cell count (cfu/ml)	Viable C. albicans cell count (cfu/ml)
0	5.83×10^6	1×10^6
2	1.83×10^8	1.16×10^6
4	3×10^8	1.33×10^6
6	7×10^8	1.067×10^6
24	1×10^9	3.5×10^3
Time (h)	Viable Orchard Maid cell count (cfu/ml)	Viable E. coli cell count (cfu/ml)
0	1.53×10^7	4×10^5
2	6.5×10^7	4.33×10^5
4	1.67×10^8	5.67×10^5
6	4×10^8	1×10^6
24	7×10^7	0

Table III.  Yakult (L. casei Shirota) versus C. albicans and E. coli in mixed culture grown statically at 37°C.

Time (h)	Viable Yakult cell count (cfu/ml)	Viable C. albicans cell count (cfu/ml)
0	1.67×10^7	5.5×10^5
2	6.5×10^7	7.5×10^5
4	1.5×10^8	2.83×10^6
6	5×10^8	1.61×10^5
24	1.5×10^9	3.5×10^3
Time (h)	Viable Yakult cell count (cfu/ml)	Viable E. coli cell count (cfu/ml)
0	4.5×10^7	1.33×10^6
2	1.67×10^8	1.67×10^6
4	5×10^8	1×10^6
6	6.5×10^8	6.67×10^5
24	4.67×10^8	0

Table IV.  D- and L-lactate production by three dairy health drink lactobacillus isolates grown in the presence of E. coli or C. albicans in mixed culture.

		E. coli		C. albicans
Product	Time (h)	D-lactate (mM)	L-lactate (mM)	D-lactate (mM)	L-lactate (mM)
Actimel	0	ND	12.3	ND	12.1
	2	ND	21.7	ND	20.7
	4	12.2	27.6	4.3	16.8
	6	7.5	41.8	8.2	22.7
	24	38.1	75.1	42	74.9
Yakult
	0	ND	36.3	6.8	16.8
	2	ND	30.7	ND	29.2
	4	8.6	38.3	7.0	30.3
	6	13.6	47.2	9.2	41.2
	24	37.5	86.7	32.9	90.7
Orchard Maid
	0	7.3	5.06	4.5	23.8
	2	7.5	37.5	6.1	18.3
	4	9.7	38.6	6.8	39.5
	6	16.9	43.9	25.1	50.9

ND, not determined.