Gbp3 is associated with the progression of lupus nephritis by regulating cell proliferation, inflammation and pyroptosis

Figures & data

Figure 1. The verification of mouse model of LN. (A) Detection of the inflammatory factor levels in serum by ELISA. (B) Detection of UCR and ALB in urine by ELISA. (C) Levels of ANA and dsDNA in serum detected by ELISA. (D) Observation of kidney histology by HE staining. E: Detection of pyroptosis-related protein levels by WB. ***P < 0.001 vs. Control. ** P < 0.01 vs. Control.

Figure 2. The exhibition of DEGs of transcriptome sequencing. (A) Volcano plot. (Red dots indicate up-regulated genes, blue dots indicate down-regulated genes and grey dots indicate non-significantly DEGs. (B): Cluster map. (Horizontal indicates genes. One column indicates each sample. Red indicates highly expressed genes and green indicates low expressed genes.)

Figure 3. Functional enrichment analysis of up-regulated DEGs. (A) Bar graph of GO enrichment analysis; (B) Bubble diagram of GO enrichment analysis; (C) Bar graph of KEGG Pathway enrichment; (D) Bubble diagram of KEGG Pathway enrichment.

Figure 4. Functional enrichment analysis of down-regulated DEGs. (A) Bar graph of GO enrichment analysis; (B) Bubble diagram of GO enrichment analysis; (C) Bar graph of KEGG Pathway enrichment; (D) Bubble diagram of KEGG Pathway enrichment.

Figure 5. qRT-PCR detection of the expression of DEGs. **P < 0.01, *** P < 0.001, vs. Control.

Table 1. The exhibition of Part DEGs.

Genes	Description	log2fc	pval	padj	Up/down
Kynu	Kynureninase	13.09495549	3.710939251	1.08703E-82	Up
Spidr	Scaffolding protein involved in DNA repair	2.874113188	1.91231E-70	1.04006E-67	Up
Gbp3	Guanylate binding protein 3	2.607491899	3.98924E-16	4.50836E-14	Up
Cbr1	Carbonyl reductase 1	2.732622864	3.29276E-66	1.59187E-63	Up
Cyp4b1	Cytochrome P450, family 4, subfamily b, polypeptide 1	−2.677624822	4.0472E–113	4.6959E-110	Down
Cndp2	CNDP dipeptidase 2 (metallopeptidase M20 family)	−2.370067605	1.02554E–88	8.11293E-86	Down

Figure 6. Gbp3 affected the occurrence of LN by regulating cell proliferation, inflammation and pyroptosis. (A) Detection the efficiency of siRNA's transfection by qRT-PCR; (B) Detection of cell proliferation by CCK-8; (C) Detection of inflammatory factors by ELISA; (D) Detection of pyroptosis-related proteins by WB. **P < 0.01, ***P < 0.001, vs. Control. ## P < 0.01, ### P < 0.001, vs. LPS.

Figure 7. Effects of Gbp3 overexpression in HGEC. (A) Gbp3 expression was detected by WB; (B) Cell viability was detected by CCK-8; (C) The levels of proinflammatory cytokines was tested using ELISA; (D) Pyroptosis-related proteins were measured by WB.

Data availability statement

The datasets generated and/or analysed during the current study are available in the [Run Yan] repository, [http://www.ncbi.nlm.nih.gov/bioproject/926917].