The long-term effect of a protein-deficient-diet enriched with vitamin B6 on the blood parameters in unexercised and exercised rats

Figures & data

Table 1. Diet composition.

Diet component	20% energy from protein	4.5% energy from protein
	g/100 g	g/100 g
Sunflower oil	0.41	0.55
Lard	5.45	5.31
Casein	18.97	4.56
Egg powder	1.61	0.20
Wheat flour	19.43	19.43
Wheat starch	30.00	30.00
Potato starch	9.14	11.36
Sugar	10.00	23.59
Mineral mix^a	4.00	4.00
Vitamin mix^b	1.00	1.00

^a100 g mineral mixture contains: 32.2 g KHPO₄, 30 g CaCO₃, 16.7 g NaCl, 10.2 g MgSO₄, 7.5 g CaHPO₄, 2.75 g FeC₆P₅O₇, 0.51 g MnSO₄, 0.08 g KJ, 0.03g CuSO₄, 0.025 g ZnCl₂, 0.005 g CoCl₂.

^b100 g vitamin mixture (in potato starch) contains: 54500IU vit. D3, 0.1 g vit. K, 0.003 g vit. B12, 1 g choline chloride, 0.101 g folic acid, 0.003 g biotin, 1.0 g inositol, 1.0 g PABA, 125000IU vit. A, 0.15 g vit. B6*, 0.25 g vit.E, 0.5 g vit. B1, 2.5 g vit. C, 0.5 g vit. PP, 0.25 g vit. B2, 2.5 g calcium panthotenate. *B6 supplemented diet –0.45 g B6/100 g vitamin mixture.

Table 2. The effect of protein-deficient diet unsupplemented or supplemented with B6 on average consumption and body weight of rats at the 60^th day of experiment in exercised or unexercised rats.

	20% of protein (I)	20% of protein + training (II)	4.5% of protein (III)	4.5% of protein + training	4.5% of protein + vit. B6 (V)	4.5% of protein + training + vit. B6 (VI)
Body weight (g)	503.7 ± 16.1^a	502.0 ± 31.9^a	199.2 ± 7.9^c	219.6 ± 9.5^c	294 ± 10.6^b	268.3 ± 7.3^b
Daily consumption (g)	25.4 ± 0.7^a	26.4 ± 0.4^a	17.6 ± 1.0^c	21.0 ± 0.3^b	21.6 ± 1.3^b	22.3 ± 0.8^b
n	n–10	n–10	n–10	n–10	n–15	n–15

Notes: Rats were fed for 60 days with: control diet (20% energy from protein, group I and II), low-protein diet (4.5% energy from protein, group III and IV) or low-protein diet (4.5% energy from protein) additionally supplemented with 300% of B6 group (V and VI). a,b,c: differences level between groups, p < .05.

Table 3. The effect of protein-deficient diet unsupplemented or supplemented with B6 on haematological parameters at 60^th day of experiment in exercised or unexercised rats.

Haematological parameters	20% of protein (I)	20% of protein + exercise (II)	4.5% of protein (III)	4.5% of protein + exercise (IV)	4.5% of protein + B6 (V)	4.5% of protein + B6 + exercise (VI)
RBC (10^6/µL)	8.9 ± 0.2^a	8.4 ± 0.2^a	9.0 ± 0.2^a	8.5 ± 0.2^a	9.6 ± 0.1^b	9.8 ± 0.1^b
HGB (g/dL)	13.2 ± 3.3^a	12.8 ± 1.6^a	12.1 ± 1.9^b	11.7 ± 3.7^b	13.3 ± 2.4^a	13.2 ± 2.5^a
HCT (%)	37.8 ± 1.1^a	37.1 ± 0.8^a	36.6 ± 1.0^a	35.2 ± 1.5^a	39.9 ± 0.7^ab	39.2 ± 0.8^b
MCV (fL)	42.6 ± 0.6^ab	44.5 ± 0.7^a	40.8 ± 0.6^b	41.2 ± 0.7^ab	41.9 ± 0.7^ab	40.0 ± 0.7^b
MCH (pg)	15.1 ± 0.4^a	15.3 ± 0.3^a	13.5 ± 0.4^b	13.6 ± 0.1^b	13.7 ± 0.2^b	13.5 ± 0.2^b
MCHC (g/L)	350 ± 10.9^a	348 ± 6.8^a	332 ± 5.8^a	333 ± 5.0^a	333 ± 6.6^a	337 ± 4.0^a
PLT (10^3/µL)	875 ± 39.7^a	941 ± 53.4^a	861 ± 94.4^a	762 ± 68.9^a	943 ± 25.1^a	840 ± 31.0^a
	n–10	n–10	n–10	n–10	n–15	n–15

Notes: Rats were fed for 60 days with: control diet (20% energy from protein, group I and II), low-protein diet (4.5% energy from protein, group III and IV) or low-protein diet (4.5% energy from protein) additionally supplemented with 300% of B6 group (V and VI). a,b: differences level between groups, p < .05.

Table 4. The effect of protein-deficient diet unsupplemented or supplemented with vitamin B6 on white blood cells (WBC) at 60^th day of experiment in exercised and unexercised rats.

WBC parameters	20% of protein (I)	20% of protein + exercise (II)	4.5% of protein (III)	4.5% of protein + exercise (IV)	4.5% of protein + B6 (V)	4.5% of protein + B6 + exercise (VI)
WBC (10^3/µl)	9.8 ± 0.7	10.7 ± 0.6	9.5 ± 0.5	16.6  ±  1.0*	12.4 ± 1.2	11.5 ± 0.9
Lymphocytes (%)	67.9 ± 2.8	71.5 ± 1.9	62.4 ± 3.6	58.9 ± 4.0	62.4 ± 2.9	60.7 ± 2.6
Neutrophils (%)	24.2 ± 2.4	19.9 ± 4.1	30.9 ± 3.7	34.9 ± 3.5	28.5 ± 2.5	30.1 ± 2.6
Eosinophils (%)	2.6 ± 0.2	2.5 ± 0.7	2.6 ± 0.4	1.5 ± 0.2	1.6 ± 0.2	1.5 ± 0.2
Monocytes (%)	5.2 ± 0.8	6.1 ± 1.0	4.1 ± 0.5	4.7 ± 0.9	7.5 ± 1.0	7.7 ± 1.0
	n–10	n–10	n–10	n–10	n–15	n–15

Note: Rats were fed for 60 days with: control diet (20% energy from protein, group I and II), low-protein diet (4.5% energy from protein, group III and IV) or low-protein diet (4.5% energy from protein) supplemented with 300% of B6 (group V and VI). *Significant differences (p < .001).

Table 5. The effect of protein-deficient diet unsupplemented or supplemented with vitamin B6 on the percentage of lymphocytes populations at 60^th day of experiment in exercised and unexercised rats.

Lymphocyte populations	20% of protein (I)	20% of protein + exercise (II)	4.5% of protein (III)	4.5% of protein + exercise (IV)	4.5% of protein + B6 (V)	4.5% of protein + B6 + exercise (VI)
Lymphocytes T (CD3^+) (%)	58.0 ± 3.6^a	49.7 ± 3.2^a	55.7 ± 3.8^a	47.3 ± 1.3^a	50.2 ± 2.6^a	45.7 ± 4.1^a
Lymphocytes B (CD45RA^+) (%)	24.4 ± 1.5^a	25.4 ± 1.9^a	22.4 ± 2.4^a	26.8 ± 1.4^a	22.8 ± 1.6^a	25.0 ± 1.2^a
Lymph T (CD4^+) (%)	36.8 ± 2.3^a	37.6 ± 2.6^a	42.0 ± 3.3^a	38.4 ± 1.3^a	41.7 ± 1.6^a	36.9 ± 1.9^a
Lymph T (CD8^+) (%)	14.2 ± 0.7^a	13.8 ± 0.5^a	12.7 ± 1.0^ab	10.4 ± 0.4^b	13.2 ± 0.8^ab	11.5 ± 0.5^ab
CD4/CD8 ratio	2.6 ± 0.3^a	2.7 ± 0.2^a	3.3 ± 0.3^ab	3.7 ±0.3^b	3.2 ± 0.3^ab	3.2 ± 0.2^ab
NK (CD161^+CD3^−) (%)	9.4 ± 0.8^a	7.6 ± 0.8^ab	4.8 ± 0.3^b	6.7 ± 0.6^ab	5.6 ± 0.6^b	7.0 ± 1.0^ab
NKT (CD161^+CD3^+) (%)	3.9 ± 0.4^a	3.1 ± 0.3^a	1.4 ± 0.2^b	1.4 ± 0.1^b	1.9 ± 0.3^b	1.7 ± 0.1^b
	n–10	n–10	n–10	n–10	n–15	n–15

Notes: Rats were fed for 60 days with: control diet (20% energy from protein, group I and II), low-protein diet (4.5% energy from protein, group III and IV) or low-protein diet (4.5% energy from protein) supplemented with 300% of B6, (group V and VI). a,b: differences level between groups, p < .05.

Table 6. The effect of protein-deficient diet unsupplemented or supplemented with vitamin B6 on serum aminotransferases activity, total protein and albumin concentration at 60^th day of experiment in exercised or unexercised rats.

	20% of protein (I)	20% of protein + exercise (II)	4.5% of protein (III)	4.5% of protein + exercise (IV)	4.5% of protein + B6 (V)	4.5% of protein + B6 + exercise (VI)
Alanine aminotransferase (UI/L)	17.3 ± 2.3^a	17.5 ± 2.1^a	18.4 ± 2.2^a	24.1 ± 3.4^a	19.1 ± 2.7^a	18.7 ± 2.5^a
Aspartate aminotransferase (UI/L)	69.0 ± 3.5^a	104.2 ± 8.2^c	100.9 ± 2.0^c	99.2 ± 1.3^bc	85.1 ± 4.3^b	90.3 ± 2.3^bc
Total protein (g/L)	81.1 ± 2.3^a	81.4 ± 2.2^a	56.3 ± 2.7^b	49.1 ± 2.4^b	59.1 ± 1.4^bc	64.1 ± 1.2^c
Albumine concentration (g/L)	38.4 ± 2.1^a	39.5 ± 1.9^a	32.9 ± 1.2^ab	30.5 ± 1.3^b	31.7 ± 1.3^b	35.2 ± 1.1^ab
	n–10	n–10	n–10	n–10	n–15	n–15

Notes: Rats were fed for 60 days with: control diet (20% energy from protein, group I and II), low-protein diet (4.5% energy from protein, group III and IV) or low-protein diet (4.5% energy from protein) a supplemented with 300% of B6 (group V and VI). a,b,c: differences level between groups, p < .05.