The occurrence of sexual Chara canescens populations (Charophyceae) is not related to ecophysiological potentials with respect to salinity and irradiance

Figures & data

Table 1. F-values and significancies for three-way ANOVA of the effects of location, irradiance, salinity and their interactions on photosynthetic parameter, chlorophyll concentration and growth rate.

		F-value
		Photosynthetic parameter			Chlorophyll			Growth
Effect	df (effect)	P max	α	E k	Chla	Chlb	Chla/b	(cm d^−1)
L	2	24.7***	17.4***	22.1***	162.3***	63.6***	16.1***	4.0*
I	1	3.8^ns	0.1^ns	2.7^ns	148.2***	82.6***	0.1^ns	20.9***
S	3	20.0***	7.4***	4.1*	0.8^ns	0.1^ns	0.5^ns	22.7***
L×I	2	4.2*	0.6^ns	2.4^ns	0.1^ns	0.9^ns	1.6^ns	0.8ns
L×S	6	9.2***	4.3**	0.5^ns	2.6*	1.7^ns	0.8^ns	2.8*
I×S	3	4.0*	1.4^ns	1.6^ns	5.0**	1.7^ns	0.6^ns	0.5^ns
L×I×S	5	6.0***	1.4^ns	1.0^ns	1.3^ns	1.8^ns	0.4^ns	0.4^ns
	df(error)	45	45	45	45	45	45	92
*p<0.05; **p<0.01; ***p<0.001. Abbreviations: df: degrees of freedom; I: irradiance; L: location; S: salinity; ns: not significant;. Units are for irradiance [µmol photons m^−2s^−1]; salinity [PSU]; P max [mmol O2 (g Chlorophyll a ^−1) h^−1]; α [mmol O2 (g Chlorophyll a ^−1) h^−1µmol photons^−1m^2s]; E k [µmol photons m^−2s^−1]; Chlorophyll [µg gFW^−1].

Fig. 1. Photosynthetic characteristics for female and male Chara canescens originating from NS and female plants collected from BS populations incubated in different irradiance – salinity regimes. The effect of location, irradiance and salinity was tested by a three-way ANOVA. Different capital letters indicate significant differences between clones and different lower case letters significant treatment effect within the locations (one-way ANOVA, Tukey post-hoc test, p<0.05).

Fig. 2. Pigment characteristics for female and male Chara canescens originating from NS and female plants collected from BS populations incubated in different irradiance – salinity regimes. The effect of location, irradiance and salinity was tested by a three-way ANOVA. Different capital letters indicate significant differences between clones and different lower case letters significant treatment effect within the locations (one-way ANOVA, Tukey post-hoc test, p<0.05).

Fig. 3. Shoot elongation of female and male Chara canescens originating from NS and female plants collected from BS populations incubated in different irradiance – salinity regimes. The effect of location, irradiance and salinity was tested by a three-way ANOVA. Different capital letters indicate significant differences between clones and different lower case letters significant treatment effect within the locations (one-way ANOVA, Tukey post-hoc test, p<0.05).