Figures & data
Figure 1. Two-dimensional reconstruction of confocal Z-series recorded from a Jurkat T cell that was stained with FITC-CTB for 15 min on ice, and fixed with 4% paraformaldehyde. Images were obtained with a Zeiss LSM510META confocal microscope. Scale bar = 10 µm.
Figure 2. A model describing the initial steps that lead to phosphorylation of the TCR in lipid rafts. In resting T cells, the TCR may weakly interact with lipid rafts. Also, Lck in these domains has low activity due to the action of the PAG/Cbp-Csk inhibitory complex. Antigen presentation by APCs strengthens the association of the TCR with lipid rafts and induces a transient redistribution of CD45 to these domains. Dephosphorylation of Lck-Y505 and of PAG/Cbp by CD45 and dissociation of Csk, increases the activity of Lck resulting in ITAM phosphorylation and signalling. This Figure is reproduced in colour in Molecular Membrane Biology online.
