Evaluation of therapeutic effect of aqueous extract of stem bark of Mangifera indica Linn. on gentamicin-induced nephrotoxicity

Figures & data

Table 1.  Effect of aqueous extract of stem bark of M. indica on SOD, lipid peroxidation, CT and RG levels.

Group	SOD levels of kidney tissue, units/mg of protein	Lipid peroxidation in kidney tissue, mM/100 mg of tissue	CT levels of kidney tissue, units/assay mixture (250 mg)	RG levels of kidney tissue, mg/100 g of tissue
1	2.494±0.12^a	24.367±0.43^b	0.988±0.02^b	133.525±3.23^d
2	1.612±0.05^a	36.400±0.95^a	0.722±0.01^d	81.337±2.97^c
3	5.914±0.13^b	15.912±0.45^d	2.964±0.02^c	183.825±2.70^b
4	10.821±0.33^c	9.688±0.87^c	5.543±0.03^a	188.487±3.75^a
Note: Means bearing the same superscript letter within the column do not differ significantly at P<0.05. All the values are expressed as Mean±SE.

Table 2.  Effect of aqueous extract of M. indica on serum creatinine (mg/dl) and urea (mg/dl) levels.

	Creatinine (mg/dl)				Urea (mg/dl)
Group	0 day	9th day	15th day	30th day	0 day	9th day	15th day	30th day
1	0.54±0.05	0.67±0.03^c	0.52±0.04^c	0.58±0.05^b	46.88±1.90	50.25±2.02^c	48.50±2.81^b	50.38±1.73^a
2	0.45±0.03	2.81±0.36^a	2.13±0.23^a	1.56±0.18^a	28.63±1.92	125.63±4.74^b	67.63±2.62^a	56.63±2.54^b
3	0.44±0.04	2.60±0.12^ab	0.89±0.02^b	0.62±0.05^b	31.88±1.88	166.25±2.99^a	41.75±2.27^bc	45.50±2.51^ac
4	0.44±0.06	2.06±0.17^b	0.88±0.03^b	0.61±0.05^b	33.25±1.89	83.75±1.94^d	44.87±2.34^b	43.63±1.59^ac
Note: Means bearing the same superscript letter within the column do not differ significantly at P<0.05. All the values are expressed as Mean±SE.

Table 3.  Effect of aqueous extract of M. indica on serum Na (mEq/L) and serum K (mEq/L) levels.

	Na (mEq/L)				K (mEq/L)
Group	0 day	9th day	15th day	30th day	0 day	9th day	15th day	30th day
1	153.19±2.81	170.86±6.89^a	162.01±1.66^b	148.94±1.54^b	192.60±1.28	149.16±2.43^a	106.87±0.64^a	80.13±0.34^b
2	172.58±2.01	170.52±3.13^a	192.14±2.59^a	166.78±5.52^ab	83.95±0.29	150.32±2.56^a	93.04±0.69^c	84.14±0.32^a
3	162.61±2.51	176.56±3.52^a	151.00±3.10^b	166.19±5.87^ab	99.68±1.64	145.87±2.84^a	108.50±0.84^ab	88.43±0.24^a
4	104.78±2.44	130.03±2.12^a	124.78±3.30^b	169.71±2.29^ab	99.44±2.67	169.54±1.82^a	102.44±0.90^bc	85.15±0.67^a
Note: Means bearing the same superscript letter within the column do not differ significantly at P<0.05. All the values are expressed as Mean±SE.

Figure 1.  Histopathological examination of normal group, GM group and aqueous stem bark extract of M. indica treated groups. Group 1 (H&E×100): (A) intact glomerulus; (B) intact proximal convoluted tubules.

Figure 2.  Histopathological examination of normal group, GM group and aqueous stem bark extract of M. indica treated groups. Group 2 (H&E×100): (A) marked glomerular changes; (B) extensive tubular necrosis; C: mononuclear cell infiltration in the interstitium.

Figure 3.  Histopathological examination of normal group, GM group and aqueous stem bark extract of M. indica treated groups. Group 3 (H&E×400): (A) vacuolar changes in proximal convoluted tubules; (B) scattered mononuclear cell infiltration in the cortex.

Figure 4.  Histopathological examination of normal group, GM group and aqueous stem bark extract of M. indica treated groups. Group 4 (H&E×400): (A) mild tubular degeneration and necrosis in the subscapular area; (B) multiple hyaline cast formation in the distal convoluted tubule; (C) pronounced dilatation of distal convoluted tubules.