Influence of prior experience with homotypic or heterotypic stressor on stress reactivity in catecholaminergic systems

Figures & data

Table I.  Comparison of changes in adrenal medulla with single and repeated IMO stress.

Single IMO	Repeated IMO
Transcription factors
↑ P-CREB, transient	↑ P-CREB
↑ c-Fos
↑ Egr1	↑ Egr1
↑ Fra-2	↑ ↑ Fra-2
Activity of MAP kinases
↑ JNK	↑ JNK
↑ Erk (1/2)
Gene expression of CA biosynthetic enzymes
	↑ TH transcription, mRNA, protein ↑ DBH transcription, mRNA, protein

Table II.  Comparison of changes in LC with single and repeated IMO stress.

Single IMO	Repeated IMO
Transcription factors
↑ P-CREB	↑ P-CREB, ↑ CREB
↑ c-Fos	↑ ↑ c-Fos
No change in Fra-2	↑ Fra-2
Activity of MAP kinases
No phosphorylation	↑ Erk (1/2)
of MAP kinases	↑ JNK1/2/3
	↑ p38
Gene expression of CA biosynthetic enzymes
↑ TH transcription, mRNA	↑ TH transcription, mRNA, protein
↑ DBH transcription, mRNA	↑ DBH transcription, mRNA, protein

Table III.  IMO triggered changes in several factors in adrenal medulla in cold pretreated animals.

	Prolonged cold (28 days)	Single IMO	Single IMO after prolonged cold
P-CREB (ser-133)	No effect	Transient rise up 3-fold with 30 min IMO	Larger rise, up 6-fold after 30 min
Egr1	No effect	Induction, up 2–3-fold with 30 min IMO	Larger more sustained rise, up 6-fold with 2 h IMO still high 3 h later
Fra-2	Up 2–3-fold	Induction up>10-fold with 2 h IMO and 3 h later rise in phospho Fra-2	Induction after 30 min, up > 10-fold with 2 h IMO and 3 h later greater rise in phospho- Fra-2