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Abstract
The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process.
| Abbreviations | ||
| CRD | = | carbonyl reactive derivatives |
| RCD | = | reactive carbonyl derivatives |
| AOPP | = | advanced oxidation protein products |
| HAS | = | human serum albumin |
| CRF | = | chronic renal failure |
| HD | = | haemodialysis |
| EPR | = | electron paramagnetic resonance |
| ANS | = | ANS-1-anilino-8-naphthalenesulfonic acid |
| bis-ANS | = | 4,4′-Bis(1-anilino-8-naphtalenesulfonic acid) |
| = | dipotassium salt | |
| FITC | = | fluorescein isothiocyanate |
| DPPH | = | 1,1-diphenyl-2-picrylhydrazyl |
| DTNB | = | 5,5′-dithio-bis (2-nitrobenzoic acid) |
| RBC | = | red blood cell |
| ROS | = | reactive oxygen species |
| TAC | = | total antioxidant capacity |
| SOD | = | superoxide dismutase |
| PBS | = | phosphate buffered saline |
| MDA | = | malondialdehyde |
| NaCl | = | sodium chloride |
| Abbreviations | ||
| CRD | = | carbonyl reactive derivatives |
| RCD | = | reactive carbonyl derivatives |
| AOPP | = | advanced oxidation protein products |
| HAS | = | human serum albumin |
| CRF | = | chronic renal failure |
| HD | = | haemodialysis |
| EPR | = | electron paramagnetic resonance |
| ANS | = | ANS-1-anilino-8-naphthalenesulfonic acid |
| bis-ANS | = | 4,4′-Bis(1-anilino-8-naphtalenesulfonic acid) |
| = | dipotassium salt | |
| FITC | = | fluorescein isothiocyanate |
| DPPH | = | 1,1-diphenyl-2-picrylhydrazyl |
| DTNB | = | 5,5′-dithio-bis (2-nitrobenzoic acid) |
| RBC | = | red blood cell |
| ROS | = | reactive oxygen species |
| TAC | = | total antioxidant capacity |
| SOD | = | superoxide dismutase |
| PBS | = | phosphate buffered saline |
| MDA | = | malondialdehyde |
| NaCl | = | sodium chloride |