Bovine Serum Albumin Nanospheres Carrying Progesterone Inclusion Complexes

Figures & data

FIG. 1. Phase solubility diagrams of progesterone in the presence of hydroxypropyl-β -cyclodextrin (A) and 2,4-dimethyl-β -cyclodextrin (B) at 10°C (▴), 25°C (▪), and 37°C (•) in distilled water.

FIG. 2. Scanning electron micrographs of the spray-dried (A) and freeze-dried nanospheres (B).

FIG. 3. (A) FT-IR spectra of Prog-HPβ CD_SD complexes: Prog (a), HPβ CD (b), Prog-HPβ CD_SD (c), and Prog-HPβ CD_FD (d). (B) FT-IR spectra of Prog-DMβ CD_FD complexes: Prog (a), DMβ CD (b), Prog-DMβ CD_SD (c), and Prog-DMβ CD_FD (d).

FIG. 4. FT-IR spectra of albumin nanospheres: Prog (a), BSA (b), Prog-BSA_SD (c), Prog-HPβ CD-BSA_SD (d), and Prog-DMβ CD-BSA_SD (e).

FIG. 5. DSC thermograms of pure drug, inclusion complexes, and nanospheres: Prog (a), DMβ CD (b), Prog-DMβ CD_SD (c), HPβ CD (d), Prog-HPβ CD_SD (e), BSA (f), Prog-DMβ CD-BSA_SD (g), and Prog-HPβ CD-BSA_SD (h).

TABLE 1 Mean diameter (nm) of Prog-β CD-BSA nanospheres calculated immediately after suspending the nanospoheres (d₀) and after 24 hr (d₂₄) and swelling degree (SD) in phosphate buffer at pH 7.4 and pH 5.5

	pH 5.5			pH 7.4
Nanospheres	d0	d24	SD	d0	d24	SD
Prog-HPβ CD-BSAFD	287,0 ± 0,2	950,0 ± 0,8	3,31	159,2 ± 0,6	432,8 ± 0,7	2,72
Prog-HPβ CD-BSASD	277,9 ± 0,5	1046,5 ± 0,2	3,76	113,9 ± 0,2	374,4 ± 0,1	3,29
Prog-DMβ CD-BSAFD	250,4 ± 0,1	625,2 ± 0,3	2,49	150,2 ± 0,4	304,6 ± 0,5	2,03
Prog-DMβ CD-BSASD	246,7 ± 0,9	798,7 ± 0,4	3,24	110,9 ± 0,3	234,0 ± 0,6	2,11

*Each value represents the mean of five experiments ± SD.

FIG. 6. Release profile of progesterone in phosphate buffer at pH 7.4 (A) and pH 5.5 (B) from the pure drug (□), Prog-HPβ CD_FD (○), Prog-DMβ CD_FD (×), Prog-HPβ CD-BSA_FD (*), Prog-DMβ CD-BSA_FD (⋄).

FIG. 7. Release profile of progesterone in phosphate buffer at pH 7.4 (A) and pH 5.5 (B) from the pure drug (□), Prog-HPβ CD_SD (○), Prog-DMβ CD_SD(×), Prog-HPβ CD-BSA_SD (*) and Prog-DMβ CD-BSA_SD(⋄).