Air-Liquid Interface Culture of Serially Passaged Human Nasal Epithelial Cell Monolayer for In Vitro Drug Transport Studies

Figures & data

FIG. 1. Change of transepithelial electrical resistance (TEER) of HNE cell monolayers grown under the LCC (•) or ALI (▴) condition. Each value is the mean ± SD of 3 determinations (passage 2).

FIG. 2. Scanning electron microscopy of the human nasal epithelial cell monolayer (passage 2). (A) ALI condition after 7 days, (B) LCC condition after 7 days, (C) ALI condition after 14 days, (D) LCC condition after 14 days, (E) and (G) ALI condition after 21 days, (F) and (H) LCC condition after 21 days. (A)–(F) are ×3000 magnification; (G) and (H) are of × 10,000 magnification.

FIG. 3. Mucin gene expression of passaged human nasal epithelial cells grown under the ALI or LCC condition for 7 or 21 days (passage 2).

FIG. 4. Transport profiles of (A) ¹⁴C-mannitol and (B) budesonide across the passage cultured human nasal cell monolayer using ALI (•) or LCC (▴) condition for 7 days (passage 2 and 3). Each value is the mean ± SD of 3 determinations.

TABLE 1 Apparent permeability coefficients (P_app) of ¹⁴C-mannitol and budesonide across the passage cultured human nasal epithelial cell monolayers grown under the air-liquid interface or liquid-covered culture condition for 7 days

	Papp (cm/s) × 10^− 6
Culture method	^14C-mannitol	Budesonide
Liquid-covered culture	0.90 ± 0.30	15.34 ± 0.66
Air-liquid interface	0.86 ± 0.04	16.53 ± 1.58

Each value is the mean ± S.D. of at least 3 determinations.