Bovine Serum Albumin–Loaded Pectinate Beads as Colonic Peptide Delivery System: Preparation and In Vitro Characterization

Figures & data

FIG. 1 Scanning electron microscopic of the pectin beads.

FIG. 2 Effect of the amount of the CaCl₂ on the particle size distribution of the beads prepared with the solution of 2% pectin.

FIG. 3 Effect of the concentration of pectin on particle size distribution of beads prepared with 10% solution of CaCl₂ and 50 mg BSA.

TABLE 1 Entrapment efficacy of different prepared formulations

Formula	Pectin concentration (%)	Cross-linking time(min)	Calcium chlorideconcentration (%)	Drug loading(mg)	Entrapment efficiency
1	2	30	10	25	98
2	2	30	10	50	99
3	2	30	10	75	91.7
4	2	30	10	100	89
5	2	30	5	50	87
6	2	30	15	50	75
7	2	30	20	50	63
8	2	15	10	50	97
9	2	60	10	50	87
10	2	120	10	50	78
11	3	30	10	50	98
12	4	30	10	50	80
13	5	30	10	50	77
14	5	30	10	50	76

FIG. 4 Effect of the amount of the albumin on the pattern of drug release.

FIG. 5 Effect of the amount of calcium chloride on the release rate of BSA from beads prepared with 3% solution of pectin containing 50 mg BSA.

FIG. 6 Effect of the amount of pectin on release of BSA from calcium pectinate beads in phosphate buffer medium (n = 4).

FIG. 7 Effect of the amount of pectin on release of BSA from calcium pectinate beads in medium containing enzyme (n = 4).

FIG. 8 Effect of cross-linking time on release of BSA from prepared beads.

FIG. 9 Effect of the presence of the pectinolytic enzymes on the pattern of BSA release from beads prepared with 5% solution of pectin and containing 50 mg BSA (n = 3).

FIG. 10 Effect of usage of glutaraldehyde on drug release from beads prepared with 5% solution of pectin in dissolution medium containing enzyme.