Figures & data
Figure 1 The response time of uric acid biosensor at 25°C (5.0 × 10−5 M uric acid) borate buffer (pH 8.0).
Figure 2 The effect of solution pH on the response to 5.0 × 10−5 M uric acid in 0.05 M borate buffer solution at 25°C. The operating potential is +0.7 V vs. Ag/AgCl.
Figure 3 The effect of temperature upon the sensitivity of biosensor against uric acid (0.05 M pH 8.0 borate buffer).
Figure 4 The effect of uric acid concentration upon the amperometric response of the biosensor (0.05 M pH 8.0 borate buffer, 25°C).
Figure 5 The calibration curve of uric acid biosensor (0.05 M, pH 8.0 borate buffer, 25°C).
Figure 6 The effect of uric acid concentration upon the amperometric response of the biosensor (Michealis-Menten plot).
Figure 7 Reuse number of the enzyme electrode (0.05 M, pH 8.0 borate buffer, 25°C).
Figure 8 Storage stabilization of the enzyme electrode (0.05 M, pH 8.0 borate buffer, 25°C).
Table 1. The properties and the optimum working conditions of the biosensor
Table 2. Interfering substances (constant uric acid concentration of 5.0 × 10−5 M) on the amperometric response of the biosensor
Table 3. Determination of uric acid in blood and urine