Figures & data
Figure 1 Oxygen binding curves of βL28N (-----), HbA (— · · —), βV67T (· · · ·), and α V63T (— — —). Buffer, pH 7.4, 50 mM Hepes + 100 mM NaCl; Temperature, 25°C.
Figure 2 Oxygen binding curves of HbA (-----) and of the mutant hemoglobin β(V1M + H2deleted + T4I + P5A + V76K) in the absence (— — —) and in the presence (· · · ·) of 100 mM Cl−. Buffer: Hepes 100 mM. Temperature, 25˚C.
Figure 3 Size exclusion chromatography of HbA (Ve 60 ml, as for a protein with MW 64 kDa) and Poly-500 (Ve 48 ml, as for a protein with MW 500 kDa).
Figure 4 Size exclusion chromatography of HbA (Ve 60 ml, as for a protein with MW 64 kDa) and Hb (Polytaur)n (Ve 38 ml = exclusion volume of the column, as for proteins with MW > 1 million).
Figure 5 Change in mean arterial blood pressure (±SD) after hypervolumetric exchange transfusion with either 5% albumin, 3% Hb Polytaur, or 3% Hb (Polytaur)n in anesthetized mice. The increase in pressure with Hb polymers was largely related to the increase in blood volume. A small portion of the increase might have been related to residual tetrameric Hb in the infusate.
Figure 6 Percent reduction in infarct volume (±SD) with hypervolumetric exchange transfusion of 3% and 6% solutions of Hb Polytaur and Hb (Polytaur)n during 2 h of middle cerebral artery occlusion in mice. Infarct volume, measured at 22 h of reperfusion, is expressed as a percent reduction from infarct volume in control groups transfused with 5% albumin.
