Evaluation of the Antioxidant Activity of Sida cordifolia.

Figures & data

Table 1.. Phytochemical analysis of Sida cordifolia.

	Extract tested
Test for	Stem	Leaves	Roots	Whole plant	Test performed
Tannins	—	—	2	—	FeCl3 test
Alkaloids	5	5	5	5	Dragendorff's test
Steroids/terpenoids	—	—	—	—	Liebermann-burchardt test
Flavonoids	2	2	3	2	Shinoda's test
Glycosides	4	4	4	4	Molisch's test
Coumarin	—	—	—	—	Hydroxylamine-HCl

The intensity of the positive test for the compound was represented in an arbitrary scoring of 1 to 5, where 5 represents the maximum amount of the compounds present.

Figure 1 Free-radical scavenging activity of Sida cordifolia. extracts, BHA, ascorbic acid, and α.-tocopherol by 1,1-diphenyl-2-picrylhydrazyl radicals. Results are mean ± SD of three parallel readings. (♦ whole plant, ▪ roots, ▴ stem, × leaves, + ascorbic acid, • α- tocopherol, □ BHA.)

Figure 2 Reducing power of Sida cordifolia. ethanol extracts, BHA, and α.-tocopherol. Results are mean ± SD of three parallel measurements. Spectrophotometric detection of the Fe⁺³–Fe⁺² transformation. (♦ whole plant, × roots, ▴ stem, ▪ leaves, • α-tocopherol, □ BHA.)

Figure 3 Superoxide anion radical scavenging activity of S. cordifolia. roots extracts and BHT by PMS-NADH-NBT method. Results are mean ± SD of three parallel measurements.

Figure 4 Inhibitory effect of Sida cordifolia. roots extract at different doses on FeCl₃-induced lipid peroxidation in rat liver homogenate. Results are mean ± SD of three parallel measurements.

Figure 5 Inhibitory effect of Sida cordifolia. roots extract at different doses on FeCl₃-induced lipid peroxidation in rat brain homogenate. Results are mean ± SD of three parallel measurements.

Figure 6 Inhibitory effect of Sida cordifolia. root extract at different doses on FeCl₃-induced lipid peroxidation in rat brain homogenate. Results are mean ± SD of three parallel measurements.

Table 2.. Antioxidant profile of Sida cordifolia.

Sr. no.	Sample tested	Conc. (µg/ml)	DPPH radical scavenging activity (%inhibition)	Anti–lipid peroxidation (%inhibition)	Superoxide anion scavenging activity (%inhibition)	Reducing power activity^a. (absorbance)
1	Whole plant	500	17.25	NR	NR	0.097
2	Root	500	50.25%	41%	55.25%	0.144
3	Stem	500	39.12%	NR	NR	0.105
4	Leaf	500	34.25%	NR	NR	0.120
5	Ascorbic acid	15	51.87%	—	—	—
6	Tocopherol acetate	500	11.75%	—	—	0.093
7	BHA	25	58.87%	—	—	0.195

^a.Increased absorbance indicates increased reducing power.