Antinociceptive Activities of Aqueous and Ethanol Extracts of Piper betle. Leaves in Rats

Figures & data

Figure 1 Effect of different doses of cold ethanol extract (CEE) of P. betle. leaves on the reaction time of rats (n = 9; hot-plate test, means ± SEM). *p < 0.05 as compared with control.

Figure 2 Effect of different doses of hot water extract (HWE) of P. betle. leaves on the reaction time of rats (n = 9; hot-plate test, means ± SEM). *p < 0.05 as compared with control.

Table 1. Effect of different doses of hot water extract (HWE) and cold ethanol extract (CEE) of P. betle. leaves on the reaction time of rats (formalin test, means ± SEM).

Treatment	n	Early phase (s)	Late phase (s)
Control (1 ml of DW)	6	71.3 ± 3.2	312.0 ± 8.9
125 mg/kg
HWE	6	67.3 ± 4.4	295.3 ± 6.1
CEE	6	59.1 ± 3.9^a.	296.6 ± 9.5
200 mg/kg
HWE	6	48.2 ± 3.9^a.	258.0 ± 4.3^a.
CEE	6	34.3 ± 5.7^a.	245.0 ± 5.9^a.
300 mg/kg
HWE	6	59.3 ± 4.0^a.	270.3 ± 4.5^a.
CEE	6	43.2 ± 3.6^a.	274.7 ± 5.1^a.
500 mg/kg
HWE	6	64.0 ± 2.4	286.6 ± 8.5
CEE	6	54.2 ± 2.8^a.	289.7 ± 6.6
Aspirin (100 mg/kg)	9	25.1 ± 1.9^a.	75.7 ± 2.8^a.

DW, distilled water.

^a.As compared with control: p < 0.05.

Table 2.. Effect of naloxone on the reaction time of rats induced by 200 mg/kg dose of cold ethanol extract (CEE) of P. betle. leaves (hot-plate test, means ± SEM).

Treatment	Reaction time (s)
200 mg/kg CEE + saline	16.2 ± 0.6
200 mg/kg CEE + naloxone	8.3 ± 0.7^a.

^a.Significant at p < 0.05.

Table 3.. Effect of metoclopramide on the reaction time of rats induced by 200 mg/kg dose of cold ethanol extract (CEE) of P. betle. leaves (hot-plate test, means ± SEM).

Treatment	Reaction time (s)
200 mg/kg CEE + 1% methyl cellulose	13.3 ± 1.1
200 mg/kg CEE + metoclopramide	14.5 ± 1.2

Not significant at p < 0.05 level.