Figures & data
Figure 1 Onset of arthritis was observed in three different strains of rats (Lewis, Wistar, and Fischer). Three strains of rats were injected with 0.1 ml of Freund's complete adjuvant into the footpad of right hind limb on day 0, and the development of arthritis was observed by measuring paw thickness on days 0, 3, 7, 11, and 14.
Figure 2 Effect of treatment with PZE at doses of 10, 20, and 40 mg/kg on paw volume on day 14 postadministration with adjuvant. Four groups of Lewis rats (5 rats per each group) were injected with 0.1 ml of Freund's complete adjuvant on day 0. Three groups of arthritic rats were treated (i.p.) with the above concentrations of PZE from day 0 to day 13. A fourth group (control) was treated with olive oil alone. Normal group did not receive any injection. Hind paw volume was measured on day 14.
Figure 3 Five groups of Lewis rats were injected with 0.1 ml of Freund's complete adjuvant on day 0 to induce AIA. First group (control) was treated with olive oil alone. The four groups the rats were treated i.p. with PZE (10, 20, 40 mg) or indomethacin daily from day 0 to day 13. Normal group did not receive any treatment. Clinical score (0–3) was measured on day 14.
Figure 4 Effect of PZE (20 mg/kg) treatment on skin thickness after cutaneous challenge with mycobacterial antigens in mineral oil (CFA). Two groups of rats were injected with 0.1 ml of Freund's complete adjuvant (CFA) and another two groups of rats were injected with 0.1 ml of incomplete Freund's adjuvant (IFA). Two groups (one group from CFA injected + one group form IFA injected) were treated with PZE from day 0 to day 13. After the rats were sacrificed on day 14, skin thickness was measured using calibrated skin fold calipers. The group that received olive oil alone served as control. Normal group did not received any injection.
Figure 5 Splenic T lymphocytes were isolated from different strains (Lewis, Wistar, Fischer) of AIA rats, PZE-treated rats, or normal rats incubated with 10 µg of Con A (amitogen) and the conditions were maintained similar to the previous culture experiments. Cultures were pulsed with 0.5 µCi of radioactive thymidine per well, and the incorporation of radioactivity was measured in scintillation counter after 18 h of incubation.
Figure 6 Five groups of Lewis rats were used. The first AIA group of rats was used as control without any treatment. The other three AIA groups of rats were treated with PZE, indomethacin (indometh) and cyclophosphamide (Cyclophos), respectively. Fifth group (control) received olive oil alone. Splenic T lymphocytes isolated from different strains of arthritic rats, PZE-treated rats, and normal rats incubated with 10 µg of Con A, and the conditions were maintained similar to the previous culture experiments. Cultures were pulsed with 0.5 µCi of radioactive thymidine per well, and the incorporation of radioactivity was measured in scintillation counter after 18 h of incubation.
