Influence of Garlic Extract on Temporal Characteristics of Lipid Peroxidation Products and Antioxidants in Tumor-Bearing Rats

Figures & data

Table 1.. Twenty-four hour biochemical determinations.

Parameters	Methods
Thiobarbituric acid reactive substances (TBARS) (in plasma)	Yagi (1978)
Reduced glutathione (in hemolysate)	Ellman (1959)
Glutathione peroxidase (in hemolysate)	Rotruck et al. (1973)
Superoxide dismutase (in hemolysate)	Kakkar et al. (1984)
Catalase (in hemolysate)	Sinha (1972)

Figure 1 Temporal patterns of TBARS in Wistar rats: (A) normal, (B) NDEA-treated, (C) NDEA+garlic-treated, (D) garlic treated. Dotted lines represent the raw data (with mean±SD) and smooth lines represent the best fitting cosinor curve (obtained using cosinorwin computer software program).

Figure 2 Twenty-four hour patterns of reduced glutathione in Wistar rats: (A) normal, (B) NDEA-treated, (C) NDEA+garlic-treated, (D) garlic-treated. Other details as in Figure 1.

Figure 3 Temporal oscillations of superoxide dismutase in Wistar rats: (A) normal, (B) NDEA-treated, (C) NDEA+garlic-treated, (D) garlic-treated. Other details as in Figure 1.

Figure 4 Rhythms of glutathione peroxidase in Wistar rats: (A) normal, (B) NDEA-treated, (C) NDEA+garlic-treated, (D) garlic-treated. Othr details as in Figure 1.

Figure 5 Temporal patterns of catalase in Wistar rats: (A) normal, (B) NDEA-treated, (C) NDEA+garlic-treated, (D) garlic-treated. Other details as in Figure 1.

Table 2.. Changes in the temporal characteristics of thiobarbituric acid reactive substances (TBARS).

Groups	Acrophase (hour)	Amplitude (nmol/ml)	Mesor (nmol/ml)	r value	p value
Normal	2306	0.2	2.1	0.65^dr	<0.001
NDEA + CC14	0602	0.3	4.2	0.17^ns	<0.5
NDEA + CC14 + garlic	0218	0.5	2.7	0.44^dr	<0.05
Garlic	2250	0.1	1.8	0.54^dr	<0.05

^drdetectable rhythmicity; ^nsnon-significant.

Table 3.. Changes in the temporal characteristics of reduced glutathione (GSH).

Groups	Acrophase (hour)	Amplitude (mg/dL)	Mesor (mg/dL)	r value	p value
Normal	2307	2.2	27.5	0.66^dr	<0.001
NDEA + CC14	1716	1.7	17.2	0.15^ns	<0.5
NDEA + CC14 + garlic	2131	2.8	21.8	0.55^dr	<0.05
Garlic	0147	2.0	35.7	0.40^dr	<0.05

^drdetectable rhythmicity; ^nsnon-significant.

Table 4.. Changes in the temporal characteristics of superoxide dismutase (SOD).

Groups	Acrophase (hour)	Amplitude (U/mg Hb^a)	Mesor (U/mg Hb^a)	r value	p value
Normal	1216	0.3	2.2	0.63^dr	<0.001
NDEA + CC14	1640	0.1	1.6	0.12^ns	<0.5
NDEA + CC14 + garlic	1450	0.2	2.0	0.42^dr	<0.05
Garlic	1000	0.3	2.8	0.56^dr	<0.05

^aamount of enzyme required to inhibit 50% of NBT reduction; ^drdetectable rhythmicity; ^nsnon-significant.

Table 5.. Changes in the temporal characteristics of glutathione peroxidase (GPx).

Groups	Acrophase (hour)	Amplitude (U mg/Hb)	Mesor (U mg/Hb)	r value	p value
Normal	1340	1.5	6.9	0.69^dr	<0.001
NDEA + CC14	2046	0.3	4.4	0.21^ns	<0.5
NDEA + CC14 + garlic	1542	1.6	6.1	0.55^dr	<0.05
Garlic	1247	0.7	9.2	0.65^dr	<0.001

^drdetectable rhythmicity; ^nsnon-significant.

Table 6.. Changes in the temporal characteristics of catalase (CAT).

Groups	Acrophase (hour)	Amplitude (U/mg Hb^a)	Mesor (U/mg Hb^a)	r value	p value
Normal	0707	0.2	2.0	0.57^dr	<0.05
NDEA + CC14	1428	0.1	1.3	0.28^ns	<0.5
NDEA + CC14 + garlic	1033	0.3	1.7	0.65^dr	<0.001
Garlic	0540	0.6	3.0	0.78^dr	<0.01

^aµmol of H₂O₂ consumed min⁻¹mg Hb⁻¹; ^drdetectable rhythmicity; ^nsnon-significant.

Yagi K (1978): Lipid peroxides and human disease. Chem Physiol Lipids 45: 3337–3351. [CSA]

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Ellman GL (1959): Tissue sulphydryl groups. Arch Biochem Biophys 82: 70–77. [PUBMED], [INFOTRIEVE], [CROSSREF]

 PubMed Web of Science ®Google Scholar

Rotruck JT, Pope AH, Ganther HE, Suransan AB, Hofeman DG, Hoekstra WG (1973): Selenium: Biochemical roles as a component of glutathione peroxidase. Science 179: 588–590. [PUBMED], [INFOTRIEVE]

 PubMed Web of Science ®Google Scholar

Kakkar P, Das B, Viswanathan PN (1984): A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21: 130–132. [PUBMED], [INFOTRIEVE], [CSA]

 PubMed Web of Science ®Google Scholar