Bioguided Fractionation of Polygonum alpinum. and Isolation and Structure Elucidation of Active Compounds

Figures & data

Figure 1 Structures of flavonoids in Polygonum alpinum..

Table 1. The effect of methanol extract of Polygonum alpinum., its fractions, and indomethacin on inflammation produced by carrageenan.

Drugs	Dose (mg/kg)	Paw volume before infl.	Paw volume after 4 h infl.	Difference volume of paw (%)	Anti-inflammation (%)	p
MeOH	25	0.81	1.22	0.41±0.050	29	<0.04
PM-1	25	0.96	1.38	0.42±0.036	27	<0.03
PM-2	25	0.80	1.20	0.47±0.039	31	<0.02
PM-3	25	0.87	1.21	0.34±0.067	41	<0.03
PM-4	25	0.90	1.27	0.37±0.045	36	<0.01
PM-5	25	0.87	1.39	0.52±0.076	10.3	> 0.5
PM-6	25	0.96	1.27	0.31±0.039	45.7	<0.003
I	25	0.97	1.10	0.13±0.053	77	<0.001
Control	–	0.83	1.41	0.58±0.41	–	–

I, indomethacin.

Table 2. The effect of pure compounds on carrageenan-induced paw edema.

Drugs	Dose (mg/kg)	Paw volume before infl.	Paw volume after 4 h infl.	Anti-inflammation (%)	p
Comp. 1	10	0.97	1.30±1.47	26.7	> 0.05
Comp. 2	10	1.10	1.52±2.30	0	–
Comp. 3	10	1.12	1.57±2.60	0	–
Comp. 4	10	1.00	1.60±1.90	0	–
Comp. 5	10	0.97	1.60±2.00	0	–
Comp. 6	10	1.17	1.72±1.80	0	–
Comp. 7	10	1.10	1.67±2.30	0	–
Comp. 8	10	1.10	1.45±1.90	22.0	–
Comp. 9	10	1.17	1.60±1.33	0	–
Control	10	1.17	1.75±2.70	0	–