Sesquiterpene Quinones from a Marine Sponge Hippospongia sp. that Inhibit Maturation of Starfish Oocytes and Induce Cell Cycle Arrest with HepG2 Cells

Figures & data

Figure 1 Structures of compounds 1-3.

Table 1.. Inhibitory activity of compounds 1 and 2/3 on proliferation of cancer cell lines.

Compound IC50 (µg/ml)
	NCI-H460	HepG2	SF-268	MCF-7	Hek-293	Hela	HL-60
l	7.1	2.5	6.3	—	6.0	2.4	*
2 and 3	10.3	5.4	*	—	11.5	4.6	*

No dose-dependent effect was observed. *-Inactive.

Table 2.. Effects of ilimaquinone (1) (10 µg/ml) on cell-cycle distribution in HepG2 cells.

Time treated (h)	G0/G1 (%)	G2/M (%)	S (%)
Control	67.2^a.	11.7	21.2
12	72.4	6.7	20.9
24	77.4	1.8	20.8
36	79.7	0	20.4

^a.The percentages of each cell cycle were analyzed by flow cytometry. Data are mean values obtained from three independent experiments.

Figure 2 Inhibitory activity of ilimaquinone (1) on oocytes maturation of starfish A: negative control; B: positive control (Vitamin E, 1 mM); C: ilimaquinone (14 µM); and D: ilimaquinone (5.6 µM).

Figure 3 The time-dependent cell cycle distribution profiles of HepG2 cells treated with 10 µg/ml of ilimaquinone (1).