Anti-inflammatory Effects of 4-Arylcoumarins in LPS-induced Murine Macrophage RAW 264.7 Cells

Figures & data

Figure 1 Chemical structures of 5,7-dimethoxy-4-p.-methoxylphenylcoumarin (1) and 5,7-dimethoxy-4-phenylcoumarin (2).

Figure 2 Evaluation of nitrite production by RAW 264.7 cells stimulated for 24 h with LPS alone or in combination with increasing concentrations (0.5–2 µg/mL) of 5,7-dimethoxy-4-p.-methoxylphenylcoumarin and (cpd 1) and 5,7-dimethoxy-4-phenylcoumarin (cpd 2). The values are the means of at least 3 determinations ±SD. Probability levels (Student's t.-test): ***p < 0.001 versus LPS-treated group.

Figure 3 Effect of 5,7-dimethoxy-4-p.-methoxylphenylcoumarin (cpd 1) on iNOS production by LPS-induced RAW 264.7 macrophage for 24 h.

Figure 4 Effect of 4-arylcoumarins on PGE₂ production in LPS-induced RAW 264.7 macrophage for 24 h. The values are the means of at least three determinations± SD. Probability level (Student's t.-test): ***p < 0.001 versus LPS-treated group.

Figure 5 Effect of 5,7-dimethoxy-4-p.-methoxylphenylcoumarin (cpd 1) and LPS-induced COX-2 expression in RAW 264.7 cells.

Figure 6 Effect of 4-arylcoumarins on LPS-induced TNF-α. production by RAW 264.7 cells. The values are the means of at least three determinations ±SD. Probability level (Student's t.-test): **p < 0.01 versus LPS-treated group.