Intrarectal Dehydroleucodine Suppresses Trinitrobenzene Sulfonic Acid–Induced Colitis in Rats, A Model for Inflammatory Bowel Disease

Figures & data

Figure 1 Structure of dehydroleucodine, isolated from Artemisia douglasiana..

Table 1.. Criteria for macroscopic scoring of colonic ulceration and inflammation.

Score	Appearance
0	Normal.
1	Localized hyperemia, no ulcers.
2	Linear ulceration without hyperemia or bowel wall thickening.
3	Linear ulceration with inflammation at one site.
4	Two or more sites of ulceration and inflammation.
5	Two or more sites of major ulceration and inflammation, or more major site of damage extending more than 1 cm along the length of the colon.
6–10	When an area of ulceration and inflammation extended more than 2 cm along the length of the colon, the score was increased by 1 for each additional centimeter of involvement.

Figure 2 Effect of DhL on area and severity of the colonic lesions induced in the rat and lesion area. Data show the mean values and the SEM of eight rats in each group. *p. < 0.05, **p. < 0.001 versus TNBS.

Figure 3 Effect of DhL on body weight gain and diarrhea. Data show the mean values and the SEM of the eight rats in each group. *p. < 0.001 versus TNBS.

Figure 4 Colonic weight of the TNBS control group and the DhL group. Data show the mean values and the SEM of the eight rats in each group. *p.<0.05 versus TNBS.

Figure 5 Micrograph of rat colon mucosa showing dilated and fused crypts with intraluminal mucus content (arrowhead) in TNBS-group. Note PMN and lymphocyte infiltration. PAS-hematoxylin, × 100.

Figure 6 Micrograph of rat colon mucosa of TNBS group. Dilated crypts (★); mitosis (arrowhead). PAS-hematoxylin, × 250.

Figure 7 Micrograph of rat colon mucosa of DhL group. Note abundant PAS + material in the crypts and ephitelial surface. PAS-hematoxylin, × 100.

Figure 8 Paraffin section of colonic mucosa of DhL group rat. Note epithelial surface with PAS + material. PAS-hematoxylin, × 400.