Antidiarrheal Activity of Lithocarpus dealbata. and Urena lobata. Extracts: Therapeutic Implications

Figures & data

Table 1. Effect of the methanol extracts of L. dealbata. seed and U. lobata. leaf on castor oil–induced diarrhea in mice.

Treatment(mg/kg, p.o.)	No. of wet feces 0–4 h	% Reduction	No. of animals devoid of wet feces at 4 h (n = 6)	%Protection
Control (2% gum acacia)	7.50±0.89	0.00	0/6	0.00
L. dealbata. extract
100	3.83±0.91^a.	48.93	1/6	16.67
200	2.67±0.95^b.	64.40	2/6	33.33
400	1.83±0.87^b.	75.60	3/6	50.00
800	1.67±1.09^c.	77.73	4/6	66.67
U. lobata. extract
100	5.00±0.37^a.	33.33	0/6	0.00
200	4.00±0.26^b.	46.67	0/6	0.00
400	1.83±0.60^c.	75.60	2/6	33.33
800	1.50±0.72^c.	80.00	3/6	50.00
Loperamide
5	0.67±0.67^c.	83.33	5/6	83.33

*Data represent mean±SEM (n = 6).

^a.,^b.,^c. p < 0.05, p < 0.01, and p < 0.001, respectively, versus. control by Student's t.-test.

Table 2. Effect of the methanol extracts of L. dealbata. seed and U. lobata. leaf on PGE₂-induced enteropooling in mice.

	PGE2 induced enteropooling
Treatment (mg/kg, p.o.)	Volume (mL) of the small intestine fluid per 100 g mouse	% Reduction
Normal control (2% gum acacia)	1.42±0.12	–
Vehicle control (2% gum acacia + PGE2)	2.93±0.10	0.00
L. dealbata. extract
100	2.58±0.10^a.	11.95
200	2.41±0.12^b.	17.75
400	2.22±0.07^d.	24.23
800	2.18±0.18^c.	25.60
U. lobata. extract
100	2.81±0.13	4.10
200	2.73±0.10	6.83
400	2.46±0.12^b.	16.04
800	2.39 ±0.16^b.	18.43
Loperamide
5	2.07±0.05^d.	29.35

*Data represent mean±SEM, n = 6.

^a,b,c,d. p < 0.05, p < 0.02, p < 0.01, and p < 0.001, respectively, versus. vehicle control by Student's t.-test.

Table 3. Effect of the methanol extracts of L. dealbata. seed and U. lobata. leaf on gastrointestinal transit in mice.

Treatment (mg/kg, p.o.)	Distance traveled by charcoal marker as % of total length of small intestine	% inhibition
Control (2% gum acacia)	76.46±2.46	0.00
L. dealbata. extract
100	71.63±2.07	6.32
200	67.00±3.60	12.37
400	64.76±2.67^b.	15.30
800	46.41±2.95^c.	39.30
U. lobata. extract
100	66.51±2.03^a.	13.01
200	54.56±3.21^c.	28.64
400	47.98±4.59^c.	37.25
800	32.16±2.10^c.	57.94
Loperamide
5	32.50±2.59^c.	57.49

*Data represent mean±SEM, n = 6.

^a,b,c. p < 0.02, p < 0.01, and p < 0.001, respectively, versus. control by Student's t.-test.

Table 4. Effect of methanol extracts of L. dealbata. seed and U. lobata. leaf on SGOT, SGPT, cholesterol, and total protein levels of experimental mice.

Treatment group (mg/kg, p.o.)	SGOT (U/L)	SGPT (U/L)	Cholesterol (mg/dL)	Total protein (g/dL)
Control (2% gum acacia)	135.33±14.39	118.00±10.38	134.33±9.92	6.73±0.54
L. dealbata. 800	159.00±14.01	162.67±9.79^b.	141.67±9.54	7.53±0.32
U. lobata. 800	147.50±9.36	132.50±11.58	136.00±5.14	7.02±0.14
Loperamide 5	189.67±14.90^a.	163.00±9.12^c.	188.33±6.00^d.	7.57±0.18

*Data represent mean±SEM, n = 6.

^a,b,c,d.p < 0.05, p < 0.02, p < 0.01, and p < 0.001, respectively, versus. control by Student's t.-test.