Hepatoprotective Effect of Crude Extract and Isolated Lignans of Justicia simplex. Against CCl₄-Induced Hepatotoxicity

Figures & data

Figure 1 Structures of simplexolin and sesamolin.

Table 1. Effect of the Justicia simplex. crude extract and isolated lignans on the biochemical parameters of CCl₄-intoxicated rats (n = 6, mean ± SEM)

Groups	Dose	ALT (U/L)	AST (U/L)	ALP (U/L)	LPO (nmol/mL)	TG (mg/dL)	Total protein (g/dL)
Control	—	20.61 ± 2.5	32.7 ± 3.2	22.56 ± 1.6	25.21 ± 2.1	52.43 ± 2.2	8.22 ± 0.31
CCl4	2 mL/kg	122.21 ± 5.2^a	152.18 ± 2.4^a	84.78 ± 1.8^a	42.31 ± 1.8^a	26.22 ± 1.8^a	5.23 ± 0.24^a
Std + CCl4	100 mg/kg	80.64 ± 3.2^b	120.24 ± 1.2^b	30.22 ± 1.8^b	23.42 ± 1.6^b	51.56 ± 1.4^b	7.92 ± 0.82^b
JSE + CCl4	100 mg/kg	72.63 ± 5.3^b	98.16 ± 3.5^b,c	30.19 ± 2.8^b	22.82 ± 1.2^b	50.32 ± 1.6^b	7.24 ± 0.12^b
Simplexolin + CCl4	10 mg/kg	68.23 ± 3.2^b,c	94.32 ± 2.1^b,c	28.02 ± 1.4^b	21.64 ± 0.8^b	49.32 ± 0.8^b	7.36 ± 0.18^b
Sesamolin + CCl4	10 mg/kg	64.5 ± 3.8^b,c	90.86 ± 2.1^b,c	24.43 ± 2.6^b	20.92 ± 1.8^b	47.22 ± 1.2^b	7.52 ± 0.09^b

a = p < 0.001 when compared with normal group; b = p < 0.001 when compared with CCl₄ groups; c = p < 0.001 when compared with standard.

Table 2. Effect of Justicia simplex. crude extract and isolated lignans on glutathione depletion and lipid peroxidation in CCl₄-intoxicated rats (n = 6, mean±SEM)

Groups	Dose	GSH (mmol/g tissue)	LPO (nmol/g of wet tissue)
Control	—	5.82 ± 2.4	362 ± 3.2
CCl4	2 mL	1.82 ± 1.2^a	594 ± 2.4^a
Std + CCl4	100 mg/kg	3.70 ± 2.0^b	372 ± 1.2^b
JSE + CCl4	100 mg/kg	2.80 ± 1.8^b,c	394 ± 3.5^b,c
Simplexolin + CCl4	10 mg/kg	2.40 ± 1.6^b,c	384 ± 2.1^b,c
Sesamolin + CCl4	10 mg/kg	2.21 ± 1.8^b,c	386 ± 2.1^b,c

a = p < 0.001 when compared with normal group; b = p < 0.001 when compared with CCl₄ groups; c = p < 0.001when compared with standard.