Cytotoxic Effects of Teucrium polium. on Some Established Cell Lines

Figures & data

Table 1. Effect of different doses of T. polium. on proliferation of some established cell lines after WST-1 assay

	A549	BT20	MCF-7	PC12
Free	0.939 ± 0.28^a	0.529 ± 0.24^a	0.269 ± 0.12^a	0.450 ± 0.14^a
DMSO	0.884 ± 0.24^a	0.337 ± 0.12^a	0.52 ± 0.09^a	0.423 ± 0.18^a
T. polium. 100	0.458 ± 0.28^b	0.183 ± 0.13^b	0.198 ± 0.16^a	0.374 ± 0.13^a
T. polium. 150	0.288 ± 0.13^b,c	0.109 ± 0.07^b	0.099 ± 0.05^b	0.296 ± 0.08^a
T. polium. 200	0.111 ± 0.09^c	0.039 ± 0.02^c	0.042 ± 0.07^c	0.077 ± 0.06^c
Taxol *	0.527 ± 0.2	0.143 ± 0.02^b	0.275 ± 0.02^a	0.709 ± 0.12^a

Data are pooled from five independent studies and are expressed as mean ± SE.

Figures with different superscripts are statistically different. a,b: p < 0.05; b,c: p < 0.01; and a,c: p < 0.001.

Tp100 = 100 µg/mL T. polium. in culture medium; Tp150 = 150 µg/mL T. polium. in culture medium; Tp200 = 200 µg/mL T. polium. in culture medium; * = 50 µg/mL Taxol in culture medium.

Table 2. Characteristics of colony-forming assays of different cell lines after treatment with 100 µg/mL T. polium..^a.

	Number of colonies			Total diameter × 10^3 (µm)			Total area × 10^3 (µm^2)
	Free	DMSO	T. polium.	Free	DMSO	T. polium.	Free	DMSO	T. polium.
A549	313	144	15	22	8	0.7	1410	408	26
BT20	162	93	14	10	4	0.6	513	149	25
MCF-7	623	209	36	75	15	2	9128	1068	90
PC12	466	359	45	36	24	2.5	2908	1373	120

^a.Data are pooled from three independent experiments.

*Significantly different from DMSO group; p < 0.05.

Figure 1 Cytotoxic effect of T. polium. on different cell lines after 14 days culture in agarose compared with DMSO goup. Data are pooled from three independent experiments. No., number of colonies; Dia., total diameter of colonies; Area, total area of colonies.