Cytotoxic Evaluation of Four Haplophyllum. Species with Various Tumor Cell Lines

Figures & data

Table 1. List of cell lines used in the cytotoxicity assay.

Cell line	Characteristics
Solid tumors
MDA-MB-453	Breast adenocarcinoma, ER −
MCF-7	Breast adenocarcinoma, ER +
SK-OV-3	Ovary adenocarcinoma
HeLa	Cervix carcinoma
LNCap-FGC-10	Prostate adenocarcinoma
5637	Bladder carcinoma
A549	Lung carcinoma
Hematopoietic cell line
Jurkat	Acute lymphoblastic leukemia
RPMI 8866	EBV transformed B-cell lymphoma
RAMOS	Burkitt lymphoma
U937	Histiocytic lymphoma

Table 2. IC₅₀ values for Haplophyllum. and Ruta. extracts tested against tumor cell lines and PBMCs.

	IC50 (µg/mL)
	H. tuberculatum.	H. stapfianum.	H. acutifolium.	H. viridulum.	R. graveolens.	C + ^a.
Jurkat	101	130	152	184	127	<7.81
RPMI8866	31.8	60.6	66.2	121	108	<7.81
RAMOS	25.3	12.3	23.7	48.3	67.2	<7.81
U937	29.3	15.6	55.9	79.0	146	<7.81
MDA-MB-453	129	146	112	379	403	<7.81
MCF-7	57.2	92.6	83.5	148	> 500	<7.81
HeLa	115	308	137	217	226	<7.81
SKOV-3	151	173	166	254	> 500	<7.81
LNCap-FGC-10	<7.81	28.3	12.2	168	27.5	<7.81
5637	23.3	54.1	64.9	121	429	<7.81
A549	405	247	277	> 500	398	<7.81
PBMCs	15.8	67.3	103	112	104	–

^a.Positive controls including doxorubicin (for Jurkat, RPMI8866, RAMOS, U937, LNCap-FGC-10, SKOV-3), vinblastine (for MDA-MB-453, MCF-7, HeLa, 5637), and etoposide (for A549).