Aphrodisiac Studies of Tricholepis glaberrima. with Supportive Action from Antioxidant Enzymes

Figures & data

Table 1.. Effect of oral administration of T. glaberrima. methanol extract on copulatory behavior in male rats.

		Mean ± SEM
Group	Parameters	0 day	7th day	14th day	21st day	28th day
Control (Vehicle)	ML	734.00 ± 67.20	656.83 ± 60.09	578.67 ± 72.61	523.67 ± 71.51	441.00 ± 87.48
	IL	993.17 ± 101.61	910.67 ± 94.81	821.00 ± 83.17	737.67 ± 56.20	667.50 ± 54.08
	MF	03.50	04.00	05.00	06.00	06.50
	IF	01.50	02.00	02.50	03.50	04.00
	PEI	724.67 ± 17.52	561.17 ± 31.34	619.83 ± 21.24	637.17 ± 46.55	676.67 ± 23.98
L-Dopa (100 mg/kg)	ML	422.50 ± 24.66**	341.33 ± 14.67**	238.17 ± 16.85**	147.67 ± 12.05**	95.50 ± 18.24**
	IL	779.67 ± 47.99	569.50 ± 44.86**	358.67 ± 17.79**	219.00 ± 17.64**	145.33 ± 13.93**
	MF	04.50*	07.00*	90.00*	12.50	14.00*
	IF	02.00	04.00*	05.00**	07.00**	09.00**
	PEI	672.67 ± 62.18	598.50 ± 33.29	523.17 ± 39.44*	551.17 ± 26.68	453.33 ± 53.67**
T. glaberrima. (200 mg/kg)	ML	328.00 ± 43.94**	197.17 ± 24.75**	116.67 ± 28.50**	90.50 ± 24.78**	55.16 ± 17.27**
	IL	646.67 ± 24.24**	472.83 ± 37.18**	262.83 ± 49.28**	172.50 ± 28.12**	92.50 ± 18.05**
	MF	05.50**	09.50**	13.00**	19.00**	23.50**
	IF	02.00	03.50**	05.00**	08.00**	11.50**
	PEI	461.17 ± 13.73**	397.17 ± 16.85* *	287.17 ± 08.58**	270.83 ± 12.06**	198.83 ± 04.67**

ML, mounting latency; IL, intromission latency; MF, mounting frequency; IF, intromission frequency; PEI, post-ejaculatory interval.

Significance level: *p < 0.05 vs. control; **p < 0.01 vs. control.

Latency results were expressed as mean ± SEM (n = 6) and numbers as medians.

Table 2.. Changes in the body weight and reproductive organs of rats after T. glaberrima. treatment.

	Body weight (g)			Weight of male reproductive organ mg/100 g b.wt (mean ± SEM)
Groups	Initial	Final	% increase	Testis	Epididymus	Vas deferens	Seminal vesicles
Control (Vehicle)	251.67 ± 7.03	263.33 ± 8.43**	4.63	569.84 ± 34.65	251.77 ± 6.53	64.56 ± 3.54	123.94 ± 15.54
L-Dopa (100 mg/kg)	265.00 ± 9.48	279.17 ± 8.58*	5.34	600.99 ± 26.24	271.35 ± 15.70	73.22 ± 4.47	125.81 ± 17.50
T. glaberrima. (200 mg/kg)	236.17 ± 7.14	287.50 ± 12.41**	21.61	641.31 ± 21.69	259.36 ± 7.76	68.55 ± 5.15	280.25 ± 26.04†

Significance level: *p < 0.05 vs. initial body weight; **p < 0.01 vs. initial body weight (paired-t.-test); †p < 0.01 vs. control.

Results were expressed as mean ± SEM (n = 6).

Table 3.. Effect on biochemical parameters and antioxidant enzymes after treatment with T. glaberrima..

			Antioxidant enzymes
Group	Alkaline phosphatase (IU/L)	Total cholesterol (mg/dL)	SOD (ng/mg)	CAT (ng/mg)
Control (Vehicle)	346.33 ± 14.14	61.00 ± 2.50	8.33 ± 1.50	252.86 ± 16.10
L-Dopa (100 mg/kg)	422.17 ± 22.24*	44.33 ± 3.76**	14.98 ± 1.60*	476.13 ± 46.94*
T. glaberrima. (200 mg/kg)	569.17 ± 58.31*	43.17 ± 2.38**	22.47 ± 3.71**	433.90 ± 49.27*

Significance level: *p < 0.05 vs. control; **p < 0.01 vs. control.

Values are mean ± SEM (n = 6).

Figure 1 Histology of the testis in a rat treated with vehicle showing the normal seminiferous tubular diameter and germinal epithelial cell thickness.

Figure 2 Histology of the testis in a rat treated with L-dopa.

Figure 3 Histology of the testis in a rat treated with methanol extract of T.glaberrima. showing the better proliferation of seminiferous tubules with a marked increase in germinal epithelium.

Table 4.. Effect of T. glaberrima. on testicular histomorphology.

Groups	Seminiferous tubular diameter (µm)	Germinal epithelial cell thickness (µm)
Control (Vehicle)	231.80 ± 2.99	58.21 ± 1.36
L-Dopa (100 mg/kg)	325.30 ± 6.11**	77.39 ± 2.05**
T. glaberrima. (200 mg/kg)	344.40 ± 7.07**	96.29 ± 3.20**

Significance level: *p < 0.05 vs. control; **p < 0.01 vs. control.

Values are mean ± SEM (n = 6).