Anticancer Activity of Steroid Alkaloids Isolated from Solanum aculeastrum.

Figures & data

Figure 1 Comparison of the growth inhibitory effects of two concentrations of tomatidine and solasodine alone and in combination (T + S) with that of cisplatin. HeLa, HT29, and MCF7 cells were exposed to 10 or 100 µM of cisplatin, tomatidine, solasodine, or the latter two combined for 48 h. Mean ± SD (n = 4). **p < 0.001; *p < 0.05 compared with control.

Table 1.. IC₅₀ values for the test compounds as obtained from log dose response curves using GraphPad Prism.

	IC50 (µM)
Cell line	Cisplatin	Tomatidine	Solasodine	Tomatidine + solasodine
HeLa	2.5	141.7	252.5	149.3
HT29	2.5	309.0	725.5	169.0
MCF7	2.5	350.1	888.0	126.9

Cells were incubated in the presence of cisplatin, tomatidine, solasodine, or a combination of the latter two for 48 h.

Table 2.. Results of cell cycle analysis using nuclear PI staining.

Treatment	Sub G1 (%)	G0/G1 (%)	S (%)	G2/M (%)
Control	1.7	55.6	19.2	23.5
Cisplatin (2.5 µM)	21.2	12.4	52.1	14.3
Tomatidine (500 µM)	1.7	64.2	18.8	15.3
Solasodine (500 µM)	2.1	66.8	16.6	14.4
Tomatidine + solasodine (100 µM each)	2.5	59.2	24.4	14.0

HeLa Cells were exposed to compounds at the indicated concentrations for 24 h before cell cycle analysis was performed.

Table 3.. Apoptosis results from annexin V–FITC/PI analysis.

Treatment	Apoptotic (%)	Necrotic (%)
Control	1.6	3.8
Cisplatin (2.5 µM)	9.6	7.0
Tomatidine (500 µM)	0.3	6.3
Solasodine (500 µM)	1.2	5.1
Tomatidine + solasodine (100 µM each)	4.2	6.6

HeLa Cells were exposed to compounds at the indicated concentrations for 24 h before the experiment was performed.