Antioxidant Profile and HPTLC-Densitometric Analysis of Umbelliferone and Psoralen in Aegle marmelos.

Figures & data

Figure 1 HPTLC chromatograms of fruit pulp of A. marmelos. extract along with standard umbelliferone and psoralen.

Figure 2 Overlay of UV absorption spectra of the marker compounds in the sample track with respective standards: (A) umbelliferone, (B) psoralen.

Table 1 Method validation parameters for the quantification of umbelliferone and psoralen by HPTLC method.

Parameters	Umbelliferone	Psoralen
Instrumental precision (% CV, n = 7)	0.83	0.91
Repeatability of standards (% CV, n = 6)	1.07	1.02
Repeatability of sample (% CV, n = 6)	1.21	1.11
Limit of detection	0.8 ng	8.0 ng
Limit of quantification	1.2 ng	16 ng
Specificity	Specific	Specific
Linearity (correlation coefficient)	0.997	0.997
Range (ng/spot)	1.6–4.8	16–96

Table 2 Recovery study of umbelliferone and psoralen by the proposed HPTLC method.

Marker compound	Amount present in the sample (μg)	Amount added (μg)	Amount found (μg)^a.	recovery (%)^a.	Average Recovery (%)
Umbelliferone	35.0	17.50	51.73 ± 2.05	98.60 ± 3.88
	35.0	35.00	69.43 ± 2.51	99.62 ± 2.93	98.88
	35.0	44.00	77.76 ± 2.80	98.44 ± 3.55
Psoralen	23.5	12.00	35.51 ± 1.32	99.99 ± 3.74
	23.5	23.50	47.33 ± 2.08	100.71 ± 4.43	100.104
	23.5	29.00	52.30 ± 2.06	99.61 ± 3.94

^a.Mean ± SD (n = 3).

Table 3 Effect of methanol extract of A. marmelos. fruit pulp on oxygen-derived free radical generation in vitro..

	Tested samples IC50 (mg/ml)^a.
Scavenging activity	Sample extract^a.	α-tocopherol acetate^a.	BHA (μ g/mL)
DPPH radical	1.015 ± 3.8	0.677 ± 2.50	42.93 ± 3.0
Nitric oxide	1.381 ± 5	0.621 ± 2.67	32.00 ± 3.9
Superoxide anion	1.102 ± 4.1	0.577 ± 2.60	43.95 ± 3.1
Lipid peroxide (liver)	1.441 ± 5.2	0.840 ± 3.52	41.50 ± 3.9
Lipid peroxide (brain)	1.628 ± 4.3	0.770 ± 5.24	58.43 ± 3.3

^a.Values are mean ± SD (n = 5). p < 0.05 versus control, Student's t.-test.

Figure 3 Reducing power of extract, α-tocopherol acetate, and BHA by spectrophotometric detection of Fe^{+ 3}-Fe^{+ 2} transformations. Results are mean ± SD of five parallel measurements.