Activity-Directed Fractionation of Pleurotus ostreatus. in the Search for Analgesics

Figures & data

Table 1 Effect of oral administration of P. ostreatus. extracts on the hot-plate reaction time of rats.^a.

	Hot-plate reaction time (s) (mean ± SEM)
		Treatment
Treatment (mg/kg)	Pretreatment	1 h	2 h	3 h	4 h	5 h
Male
Control: H2O (n = 6)	7.8 ± 0.4	7.7 ± 1.0	4.9 ± 0.4	5.5 ± 0.8	5.7 ± 1.0	5.1 ± 0.4
Control: 5% DMSO (n = 6)	9.7 ± 0.9	9.4 ± 0.5	8.8 ± 0.9	8.5 ± 0.9	7.5 ± 0.7	6.5 ± 0.4
500 (n = 6) M-A	9.0 ± 1.4	15.1 ± 2.7	10.6 ± 2.5	8.8 ± 1.6	7.7 ± 2.2	7.0 ± 1.4
500 (n = 6) M-D	7.8 ± 0.5	7.7 ± 0.8	7.3 ± 0.9	7.3 ± 0.4	6.6 ± 0.8	6.3 ± 1.0
500 (n = 6) M-H	8.1 ± 0.8	7.4 ± 0.4	5.9 ± 0.9	7.5 ± 1.0	6.0 ± 0.8	5.7 ± 0.8
Female
Control: H2O (n = 9)	6.8 ± 0.6	7.6 ± 0.7	8.0 ± 0.9	7.1 ± 0.6	6.8 ± 0.5	7.1 ± 0.4
500 (n = 9) FD.-A	6.3 ± 0.6	9.7 ± 1.3	7.0 ± 0.5	6.9 ± 0.8	6.9 ± 0.7	7.0 ± 0.9

^a.Males and females in the diestrous stage are abbreviated as M and F_D, respectively. The extract used in each experiment is given after the hyphen as A, D, and H, for acetone, dichloromethane, and hexane, respectively. The acetone extract was dissolved in water, and the dichloromethane and hexane extracts were dissolved in 5% DMSO.

*p < 0.05 compared with the respective pretreatment.

Table 2 Effect of naloxone on the hot-plate reaction time of male rats, induced by 500 mg/kg of acetone extract of P. ostreatus..

	Reaction time on hot-plate test (s) (mean ± SEM)
Treatment (mg/kg)	Pretreatment	First hour
500 (n = 6) no naloxone	6.7 ± 0.6	10.8 ± 0.9
500 (n = 6) with naloxone	8.3 ± 0.6	8.8 ± 0.7

*Values are significant at p < 0.05.

Table 3 Effect of oral administration of fractions of the acetone extract on the hot-plate reaction time of rats.^a.

	Hot-plate reaction time (s) (mean ± SEM)
		Treatment
Treatment (500 mg/kg)	Pretreatment	1 h	2 h	3 h	4 h	5 h
Male
AqFrA-M (n = 6)	6.8 ± 0.5	9.3 ± 1.2	8.2 ± 1.3	7.6 ± 1.3	5.2 ± 0.8	5.1 ± 0.8
EAFrA-M (n = 6)	7.3 ± 0.4	8.5 ± 1.0	7.6 ± 0.8	6.3 ± 0.9	6.4 ± 1.1	6.2 ± 0.9
DMFrA-M (n = 6)	6.5 ± 0.6	6.6 ± 0.8	6.9 ± 0.7	6.6 ± 0.6	5.2 ± 0.7	5.3 ± 0.7
Female
AqFrA-FD.(n = 9)	9.3 ± 0.7	11.7 ± 0.8	10.0 ± 0.8	7.1 ± 0.9	5.7 ± 0.6	6.3 ± 0.6

^a.Males and females in the diestrous stage are abbreviated as M and F_D, respectively. The fractions obtained from solvent partition of acetone extract to dichloromethane, ethyl acetate, and aqueous are indicated as DMFrA, EAFrA, and AqFrA, respectively.

*Values are significant at p < 0.05 compared with pretreatment.

Table 4 Effect of oral administration of AqFr-1, AqFr-2 and AqFr-3 on the hot-plate reaction time of male rats.

	Hot-plate reaction time (s) (mean ± S.E.M.)
		Treatment
Treatment (500 mg/kg)	Pretreatment	1 h	2 h	3 h	4 h	5 h
Male
AqFrA-1 (n = 6)	8.1 ± 0.9	10.2 ± 0.7	8.4 ± 0.8	6.8 ± 0.7	6.0 ± 0.5	6.3 ± 0.5
AqFrA-2 (n = 6)	8.1 ± 1.1	13.7 ± 3.3	11.2 ± 2.8	7.5 ± 0.7	6.4 ± 1.0	6.0 ± 0.4
AqFrA-3 (n = 6)	8.0 ± 1.1	16.1 ± 1.2**	12.0 ± 1.6	13.8 ± 0.8	10.1 ± 1.5	9.0 ± 0.9
Morphine (n = 6): 15 mg/kg	7.5 ± 0.5	13.8 ± 0.8**	16.7 ± 1.5**	11.5 ± 2.6	12.6 ± 1.4**	9.4 ± 1.4

Values are significant at **P < 0.01 and at