Antimicrobial Activity of Ethanol Extract from Leaves of Casearia sylvestris.

Figures & data

Figure 1 Chromatographic profile of elution of ethanol extract from leaves of C. sylvestris. using Sephadex LH-20 column. The peak III showed antimicrobial activity against Escherichia coli. and Candida albicans..

Figure 2 The chromatographic profile of fractions elution, corresponding with peak III (see Fig. 1) using RP-HPLC. Peaks 2 and 3 showed activity against Escherichia coli. and Candida albicans..

Figure 3 Structures of isobutyl gallate-3,5-dimethyl ether (IGDE) and methyl gallate-3,5-dimethyl ether (MGDE).

Table 1 The minimal inhibitory concentration^a. of IGDE and MGDE (in μ g/mL) against bacteria and fungi.

	IGDE	MGDE
Microorganism	MIC (μ g/mL)	MIC (μ g/mL)
Gram-negative bacteria
Escherichia coli.	156.0^b.	312.0
Salmonella enteritidis.	156.0	156.0
Shigella sonnei.	−^c.	−
Pseudomonas aeruginosa.	312.0	624.0
Serratia marcescens.	−	−
Klebsiella pneumoniae.	−	−
Gram positive bacteria
Enterococcus faecalis.	9.8	39.0
Staphylococcus aureus.	9.8	39.0
Yeast
Candida albicans.	78.0	−
Candida tropicalis.	156.0	−
Candida guilliermondii.	156.0	−
Filamentous fungi
Aspergillus flavus.	−	−
Aspergillus niger.	−	−

^a. Method for determination of MIC of IGDE and MGDE from leaves of C. sylvestris.: agar diffusion.

^b.Last concentration that caused inhibition in bacterial growth.

^c.(−) Presence bacterial growth = inhibitory activity negative.