In Vitro Anthelmintic Effects of Medicinal Plants Used in Czech Republic

Figures & data

Table 1.  Ethnobotanical data on medicinal plants.

Botanical name and voucher specimen number	Family	Czech names	Parts used	Ethnomedicinal uses^a
Allium sativum L. (Urb 004)	Alliaceae	Česnek setý	Bulbs	Anthelmintic effect, arteriosclerosis, hypertensive diseases, cardiac complaint, bowel diseases
Artemisia absinthium L. (Urb 009)	Asteraceae	Pelyněk pravý	Aerial part	Anthelmintic effect, dysorexia, enteralgia, biliousness
Artemisia vulgaris L. (Urb 003)	Asteraceae	Pelyněk Černobýl	Aerial part	Antiparasitic effect, dysorexia, stomachache, dysmenorhea
Carum carvi L. (Urb 015)	Apiaceae	Kmín kořenný	Fruit	Antiparasitic and bacteriostatic effect, dysorexia, stomachache
Consolida regalis Gray (Urb 002)	Ranunculaceae	Ostrožka stračka	Flowers	Anthelmintic effect, cough
Cucurbita pepo L. (Urb 011)	Cucurbitaceae	Tykev turek	Seeds	Anthelmintic effect, ureteritis
Daucus carota L. (Urb 007)	Apiaceae	Mrkev obecná	Roots	Antiparasitic effect, liver complaint, enteritis, jaundice, and ureteritis
Dryopteris filix-mas (L.) Schott (Urb 014)	Dryopteridaceae	Kaprad’ samec	Rhizomes	Antiparasitic effect, fungal infections
Erigeron canadensis L. (Urb 005)	Asteraceae	Turan kanadský	Flowering aerial part	Anthelmintic and antiparasitic effect, hemorrhage, bloody diarrhea, ureteritis
Hedera helix L. (Urb 010)	Araliaceae	Břečt'an popínavý	Leaves	Antiparasitic effect, cough, bronchitis, wounds
Inula helenium L. (Urb 016)	Asteraceae	Oman pravý	Rhizomes and roots	Anthelmintic effect, bronchitis, wounds
Juglans regia L. (Urb 001)	Juglandaceae	Ořešák královský	Pericarp	Anthelmintic effect, exanthesis, fungal infections
Satureja hortensis L. (Urb 013)	Lamiaceae	Saturejka zahradní	Aerial part	Anthelmintic effect, gastritis, enteritis
Tanacetum vulgare L. (Urb 008)	Asteraceae	Kopretina vratič	Aerial part	Anthelmintic effect, articular diseases
Thymus vulgaris L. (Urb 012)	Lamiaceae	Mateřídouška tymián	Aerial part	Antiparasitic effect, bronchitis, gastritis, dyspepsia, flatulence, diarrhea, wounds
Valeriana officinalis L. (Urb 006)	Valerianaceae	Kozlík lékařský	Rhizomes	Anthelmintic effect, bowel neurosis, insomnia, heart distress

^aThe ethnomedical information has been taken from Korbelar et al. (1978).

Table 2.  Ovicidal effect (o) of ethanol extracts from some species of Czech medicinal plants.

		Concentration of extracts (μg/mL)
Species and controls	Yield^a (%)	62.5	125	250	500	1000	2000
		O ± SD (%)
A. sativum	12.67	64.94 ± 9.09	54.53 ± 0.22	57.86 ± 4.46	59.37 ± 13.27	53.46 ± 5.61	38.44 ± 5.15
A. absinthium	20.07	65.95 ± 8.38	63.79 ± 9.39	60.44 ± 2.41	65.55 ± 1.65	64.67 ± 1.28	67.25 ± 7.32
A. vulgaris	13.33	76.93 ± 2.62	70.98 ± 3.42	68.24 ± 3.44	65.03 ± 4.40	61.40 ± 3.51	63.68 ± 7.10
C. carvi	7.87	60.16 ± 3.67	64.02 ± 2.59	58.10 ± 5.24	63.21 ± 5.07	59.15 ± 1.18	57.95 ± 0.69
C. regalis	25.20	91.90 ± 2.13	89.00 ± 2.22	86.67 ± 3.24	82.05 ± 1.15	81.54 ± 3.25	76.56 ± 2.02
C. pepo	17.60	85.03 ± 9.02	82.52 ± 7.54	76.08 ± 11.06	72.64 ± 2,31	70.83 ± 8.91	69.43 ± 5.43
D. carota	12.27	70.50 ± 3.14	69.01 ± 1.43	70.50 ± 4.11	64.79 ± 2.11	59.77 ± 1.28	59.80 ± 4.43
D. filix-mas	28.40	97.39 ± 0.59	94.79 ± 2.09	88.48 ± 0.78	83.09 ± 0.39	78.83 ± 0.33	69.51 ± 0.40
E. canadensis	18.58	81.61 ± 2.95	84.29 ± 1.16	73.74 ± 7.18	76.59 ± 3.88	76.33 ± 2.46	70.65 ± 2.12
H. helix	27.55	91.13 ± 3.40	71.40 ± 2.32	74.35 ± 3.13	68.97 ± 8.72	63.70 ± 5.71	60.65 ± 3.50
I. helenium	18.40	84.10 ± 2.55	77.03 ± 2.93	69.48 ± 3.18	69.64 ± 3.68	66.37 ± 1.21	63.12 ± 3.36
J. regia	29.27	66.76 ± 0.67	64.33 ± 2.73	64.23 ± 5.69	62.73 ± 1.18	61.05 ± 2.24	52.97 ± 2.89
S. hortensis	16.47	90.69 ± 4.22	84.86 ± 1.87	86.91 ± 3.73	73.64 ± 1.45	69.00 ± 9.68	68.74 ± 1.35
T. vulgare	23.07	76.57 ± 4.58	74.77 ± 7.22	70.55 ± 6.50	66.79 ± 4.18	58.39 ± 3.86	54.02 ± 3.61
T. vulgaris	12.60	93.11 ± 2.40	90.54 ± 6.29	76.41 ± 9.58	76.29 ± 17.96	68.53 ± 13.47	58.14 ± 6.06
V. officinalis	18.80	83.24 ± 2.59	64.66 ± 9.81	61.86 ± 2.08	62.39 ± 4.86	62.38 ± 2.69	60.68 ± 5.23
Zentel^b		10.16 ± 1.99	10.13 ± 1.28	7.97 ± 1.17	9.83 ± 4.41	7.90 ± 1.65	7.12 ± 3.88
PBS with DMSO ^c		80.73 ± 5.81	80.73 ± 5.81	80.73 ± 5.81	80.73 ± 5.81	80.73 ± 5.81	80.73 ± 5.81

Biological material: helminths, eggs from Ascaris suum. Parts used are provided in Table 1.

O: ovicidal effect (O) = M/T × 100. SD (%), standard deviation. T, total number of eggs deposited in individual wells; M, the ratio between the number of larvae.

^aResidue of the extract in terms of starting material.

^bAlbendazole, positive control.

^cNegative control, 2% DMSO in PBS solution (pH 7.2, 0.15 M).

Table 3.  Larval migration inhibition (LMI) of ethanol extracts from some species of Czech medicinal plants.

	Concentration of extracts (μg/mL)
Species and controls	62.5	125	250	500	1000	2000
	LMI ± SD (%)
A. sativum	48.72 ± 11.59	55.00 ± 2.89	58.33 ± 8.56	61.29 ± 3.89	63.41 ± 3.89	66.67 ± 12.94
A. absinthium	35.00 ± 12.80	37.78 ± 7.07	48.49 ± 8.37	60.00 ± 4.44	61.76 ± 4.17	76.92 ± 2.64
A. vulgaris	24.20 ± 8.70	27.94 ± 8.38	32.91 ± 17.48	33.33 ± 4.11	38.84 ± 8.71	42.65 ± 7.17
C. carvi	29.63 ± 4.20	23.81 ± 18.78	36.67 ± 25.17	40.91 ± 11.30	40.00 ± 16.25	38.18 ± 3.05
C. regalis	30.71 ± 4.60	30.34 ± 13.01	46.88 ± 19.87	42.86 ± 9.82	53.49 ± 1.89	51.67 ± 3.21
C. pepo	39.13 ± 13.30	30.00 ± 4.28	37.50 ± 17.89	41.67 ± 19.76	45.00 ± 14.46	48.28 ± 2.52
D. carota	41.94 ± 18.00	48.78 ± 22.22	52.17 ± 17.77	57.14 ± 20.20	57.89 ± 12.13	58.33 ± 16.00
D. filix-mas	25.45 ± 6.16	30.77 ± 15.98	40.68 ± 4.33	37.35 ± 6.28	48.72 ± 8.35	66.67 ± 16.92
E. canadensis	23.81 ± 20.09	26.32 ± 18.54	35.29 ± 21.47	38.46 ± 16.07	35.71 ± 21.73	39.13 ± 24.17
H. helix	21.95 ± 6.83	25.00 ± 25.26	25.93 ± 11.52	29.41 ± 4.21	27.03 ± 2.64	28.57 ± 7.74
I. helenium	48.10 ± 4.52	48.33 ± 3.65	41.18 ± 6.94	55.56 ± 18.32	48.48 ± 13.96	68.75 ± 2.81
J. regia	27.94 ± 0.75	40.00 ± 8.22	43.55 ± 2.83	47.76 ± 23.46	53.66 ± 2.98	58.97 ± 20.36
S. hortensis	47.02 ± 9.32	48.19 ± 21.04	50.00 ± 14.04	53.33 ± 12.32	52.38 ± 7.14	60.00 ± 26.90
T. vulgaris	25.14 ± 4.59	34.78 ± 8.82	35.19 ± 14.81	35.21 ± 23.12	35.71 ± 26.81	40.40 ± 16.12
T. vulgare	17.95 ± 13.41	27.44 ± 10.50	31.97 ± 16.91	37.50 ± 22.16	45.45 ± 11.98	49.15 ± 11.57
V. officinalis	50.00 ± 0.00	50.00 ± 22.36	48.61 ± 15.12	61.22 ± 2.45	62.50 ± 14.66	87.50 ± 29.32
Zentel^a	31.25 ± 0.58	38.78 ± 6.28	41.30 ± 3.08	50.00 ± 3.65	62.75 ± 6.85	78.26 ± 4.82
PBS with DMSO^b	15.86 ± 5.25	15.86 ± 5.25	15.86 ± 5.25	15.86 ± 5.25	15.86 ± 5.25	15.86 ± 5.25

Biological material: helminths, exsheathed L3 larvae from Trichostrongylus colubriformis. Parts used and yields are provided in Tables 1 and 2, respectively.

LMI, larval migration inhibition (%) = [(T−M)/T] × 100. SD (%), standard deviation. T, total number of L3 deposited in individual wells; M, number of L3 having migrated through (activity move).

^aAlbendazole, positive control.

^bNegative control, 2% DMSO in PBS solution (pH 7.2, 0.15 M).

J Korbelar, Z Endris, and J Krejca. (1978). Nase rostliny v lekarstvi. Avicenum, Zdravotnicke nakladatelstvi, Praha

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