Antihyperglycemic Activity of Pongamia pinnata Stem Bark in Diabetic Mice

Figures & data

Table 1.  Effect of PPSBAE on serum glucose level in alloxan-induced diabetic mice (acute study).

	Mean fasting serum glucose level (mg/dl, SEM)
Treatment (mg/kg, p.o.)	0 h	1 h	2 h	4 h	6 h	24 h
Vehicle (gum acacia 2%)	509.23 ± 18.13	507.58 ± 16.74	509.45 ± 19.09	509.00 ± 17.34	510.36 ± 19.45	512.25 ± 20.58
Glyburide (10)	539.84 ± 19.87	498.25 ± 34.47	458.21± 16.14*	419 ± 15.54*	358.86 ± 12.10**	521.71 ± 34.27
PPAlex (100)	482.77 ± 24.72	434.01 ± 28.99	420.41 ± 35.71	406.15 ± 29.72*	399.02 ± 24.07**	460.18 ± 30.27
PPAlex (200)	532.10 ± 23.90	442.59 ± 35.47	401.78 ± 22.61*	380.31 ± 22.01**	370.57 ± 24.10**	506.22 ± 27.96
PPAlex (400)	526.17 ± 24.74	444.28 ± 21.25	381.28 ± 29.13**	296.90 ± 23.68**	305.99 ± 22.23**	402.42 ± 26.05*

Values are mean ± SEM, n = 6 in each group; Statistical analysis by one-way ANOVA followed by post hoc Dunnett's test using Graphpad Instat software; P value * < 0.05,** < 0.01 compared to vehicle treated group (gum acacia 2%, 10 ml/kg).

Table 2.  Effect of PPSBAE on serum glucose level in alloxan-induced diabetic mice (subacute study).

	Mean fasting serum glucose level (mg/dl, SEM)
Treatment (mg/kg, p.o.)	Day 0	Day 7	Day 14	Day 21	Day 28
Vehicle (gum acacia 2%)	509.23 ± 18.13	506.04 ± 17.69	510.03 ± 18.34	510.69 ± 18.64	509.45 ± 17.89
Glyburide (10)	539.84 ± 19.87	467.54 ± 14.51**	397.61 ± 17.55**	364.85 ± 14.83**	296.25 ± 18.45**
PPAlex (100)	482.77 ± 24.72	379.69 ± 15.79**	348.42 ± 30.63**	329.72 ± 28.76**	271.82 ± 15.15**
PPAlex (200)	532.10 ± 23.90	408.36 ± 25.89**	334.91 ± 29.25**	307.98 ± 30.23**	255.90 ± 21.13**
PPAlex (400)	526.17 ± 24.74	371.40 ± 21.33**	326.11 ± 20.34**	275.20 ± 17.78**	221.75 ± 14.68**

Values are mean ± SEM, n = 6 in each group; Statistical analysis by one-way ANOVA followed by post hoc Dunnett's test using Graphpad Instat software; P value * < 0.05, ** < 0.01 compared to vehicle treated group (gum acacia 2%, 10 ml/kg).

Table 3.  Effect of PPSBAE on body weight in alloxan-induced diabetic mice.

	Body weight (g ± SEM)
Treatment (mg/kg, p.o.)	Day 0	Day 7	Day 14	Day 21	Day 28
Vehicle (gum acacia 2%)	32.67 ± 0.75	27.33 ± 0.91	22.83 ± 0.79	19.17 ± 0.87	16.83 ± 0.65**
Glyburide (10)	30.33 ± 0.95	30.67 ± 0.91	30.83 ± 1.19**	31.17 ± 1.92**	30.67 ± 2.07**
PPAlex (100)	29.5 ± 1.05	28.5 ± 1.43	28.33 ± 1.51*	26.67 ± 1.05**	24.5 ± 1.23**
PPAlex (200)	33.5 ± 1.28	31± 1.63	32.33 ± 1.51**	30.83 ± 1.05**	30.67 ± 1.23**
PPAlex (400)	31.5 ± 1.02	31.5 ± 0.91	30.6 ± 1.43**	31± 1.43**	31.17 ± 1.43**

Values are mean ± SEM, n = 6 in each group; Statistical analysis by one-way ANOVA followed by post hoc Dunnett's test using Graphpad Instat software; P value * < 0.05, ** < 0.01 compared to vehicle treated group (gum acacia 2%, 10 ml/kg).

Table 4.  Effect of PPSBAE on oral glucose tolerance test in non-diabetic mice.

	Mean fasting serum glucose level (mg/dl, SEM)
Treatment (mg/kg, p.o.)	Before glucose	0 min	30 min	60 min	120 min
Vehicle (gum acacia 2%)	107.67 ± 5.14	284.99 ± 3.70	231.42 ± 9.98	189.40 ± 8.10	170.68 ± 10.97
Glyburide (10)	114.40 ± 4.82	286. 89 ± 6.55	245.62 ± 15.47	197.19 ± 14.16	166.71 ± 9.46
PPAlex (200)	100.68 ± 3.41	252.98 ± 21.12	229.13 ± 19.54	146.23 ± 11.73*	108.47 ± 10.03**

Values are mean ± SEM, n = 6 in each group; Statistical analysis by one-way ANOVA followed by post hoc Dunnett's test using Graphpad Instat software; P value * < 0.05, ** < 0.01 compared to vehicle treated group (gum acacia 2%, 10 ml/kg).