Anti-Helicobacter pylori activities of Eucalyptus grandis: Effects on susceptibility, urease activity and cell surface hydrophobicity

Figures & data

Table 1.  Antimicrobial susceptibility of Helicobacter pylori to methanol extracts of Eucalyptus grandis. Diameter of zones of inhibition (mm) and MICs.

H. pylori strains	Methanol extract (μg/ml)			MICs of extract μg/ml	Pylorid® 25 μg/ml	Bismuth 25 μg/ml	50% Methanol
	100	50	6.5
					31 ± 0.4	30 ± 0.1
ATCC 24376	30 ± 0.3	24 ± 0.1	14 ± 0.3	0.39	18 ± 0.3	20 ± 0.2	0
UCH 97001	34 ± 0.3	28 ± 0.1	14 ± 0.3	100	R	R	0
UCH 97002	R	R	R	1.56	20 ± 0.3	22 ± 0.2	0
UCH 97009	20 ± 0.1	16 ± 0.3	10 ± 0.4	100	R	R	0
UCH 98020	12 ± 0.2	R	R	0.78	17 ± 0.2	18 ± 0.3	0
UCH 98026	18 ± 0.3	14 ± 0.1	11 ± 0.3	1.56	26 ± 0.3	24 ± 0.3	0
UCH 99039	15 ± 0.1	12 ± 0.3	9 ± 0.1	0.78	18 ± 0.2	20 ± 0.2	0
UCH 99041	20 ± 0.1	14 ± 0.1	10 ± 0.4	1.56	17 ± 0.4	19 ± 0.3	0
UCH 99045	15 ± 0.3	13 ± 0.4	9 ± 0.2	0.78	20 ± 0.2	22 ± 0.2	0
UCH 99050	14 ± 0.3	14 ± 0.1	11 ± 0.3	1.56	25 ± 0.3	27 ± 0.2	0
UCH 99052	18 ± 0.4	15 ± 0.1	10 ± 0.3	1.56			0

*Result is average of triplicate experiment.

R: Resistant; Diameter of cork borer = 8 mm.

Table 2.  Effect of methanol extracts of Eucalyptus grandis on the cell surface hydrophobility of Helicobacter pylori strains by SAT method.

(NH4)2 SO4 conc. (M)	ATCC 43504	UCH 97001	UCH 97002	UCH 97009	UCH 98020	UCH 98026	UCH 99039	UCH 99041	UCH 99045	UCH 99050	UCH 99052
0.10M	-	-	-	-	-	-	-	-	-	-	-
0.20M	-	-	-	-	-	-	-	-	-	-	-
0.25M		-	-	-	-	-	-	-	-	-	-
0.30M	-	-	-	-	-	-	-	-	-	-	-
0.40M	-	-	-	-	-	-	-	-	-	-	-
1.0M	-	+	-	-	-	-	-	-	-	-	-
1.5M	+	+	-	+	-	+	-	+	-	-	-
2.0M	+	+	-	+	-	+	-	+	-	-	-
3.0M	+	+	-	+	-	+	-	+	+	-	+
SAT TITERS	≤1.5	≤1.0	>3	≤1.5	>3	≤1.5	>3	≤1.5	<3	>3	<3

* Result is average of triplicate experiment.

- No aggregation (negative SAT); + Aggregation (positive SAT).