Antimicrobial activity of Alepidea amatymbica

Figures & data

Table 1.  Antibacterial activity of the acetone and methanol extracts of the leaf, stem, rhizome and root of Alepidea amatymbica.

Bacterial species	Gram +/-	Minimum inhibitory concentration (mg mL^−1)
		Acetone				Methanol
		Leaf	Stem	Root	Rhizome	Leaf	Stem	Root	Rhizome
Bacillus aereus	+	na	na	5	5	na	na	10	5
Staphylococcus epidermidis	+	na	na	na	7.5	na	na	na	10
Staphylococcus aureus	+	7.5	7.5	5	2.5	10	10	10	5
Micrococcus kristinae	+	na	na	na	na	na	na	na	na
Streptococcus pyrogens	+	na	10	na	na	na	10	na	na
Escherichia coli	-	na	na	na	na	na	na	na	na
Salmonella pooni	-	na	na	na	na	na	na	na	na
Serratia marcescens	-	na	na	na	na	na	na	na	na
Pseudomonas aeruginosa	-	na	na	na	na	na	na	na	na
Klebsiella pneumonae	-	na	na	na	na	na	na	na	na

na: no activity at corresponding bacteria and extracts.

Table 2.  Antifungal activity of the acetone and methanol extracts of the leaf, stem, rhizome and roots of Alepidea amatymbi.

Concentration (mg/mL)	Growth inhibition (%)
	Leaf			Stem			Rhizome			Root
	Af	An	Pn	Af	An	Pn	Af	An	Pn	Af	An	Pn
Acetone
5	47.5^d	46.21^d	56.23^d	57.17^e	56.41^d	62.05^e	56.95^e	65.21^e	76.34^e	58.89^d	51.39^d	62.5^c
1	26.95^c	25.61^c	23.08^c	38.18^d	51.82^d	35.50^d	44.17^d	59.72^d	61.67^d	39.44^c	29.17^c	51.94^c
0.5	10.83^b	9.85^b	21.77^c	32.72^c	24.44^c	22.50^c	35.09^c	33.61^c	35.00^c	23.06^b	22.78^c	36.94^b
0.1	2.5^a	2.26^a	10.49^b	25.56^b	16.39^b	15.32^b	25.56^b	23.89^b	15.00^b	16.11^b	7.50^b	6.67^a
Acetone Cont.*	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a
PDA Control	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a
LC50	>5	>5	4.25	3.49	0.97	3.18	2.82	0.81	0.78	3.17	4.75	0.94
Methanol
5	54.44^e	62.80^e	57.22^d	55.28^d	53.96^d	66.39^e	56.39^e	66.52^d	81.11^d	56.11^e	51.95^d	61.78^d
1	29.45^d	41.39^d	23.33^c	36.67^c	51.95^d	33.33^d	36.67^d	57.5^d	68.05^c	48.29^d	24.91^c	39.44^c
0.5	21.67^c	23.33^c	22.50^c	31.11^b	25.95^c	26.67^c	31.11^c	30.00^c	30.84^b	33.61^c	16.39^b	23.61^b
0.1	6.67^c	8.96^b	10.56^b	27.22^b	8.61^b	21.67^b	26.67^b	12.22^b	24.17^b	6.67^b	6.96^a	18.33^b
Methanol Cont.	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a
PDA Control	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a	0.00^a
LC50	4.29	2.61	4.15	3.87	0.93	0.77	3.7	0.86	3.02	1.87	4.71	2.89

Values are means of percentage growth inhibition of three replicates. Values within a column of a treatment followed by the same superscript are not significantly different at P < 0.05 according to the LSD test. Af: Aspergillus flavus; An: Apergillus niger; Pn: Penicillium notatum. LC₅₀: values in mg mL⁻¹ were calculated by extrapolation. Cont.*: Control.