Effects of Myristica fragrans oil on the reproductive organs of male albino rats

Figures & data

Figure 1.  Showing the Chemical structure of Myristicin.

Table 1.  Body and organs weight of Myristica fragrans oil treated male albino rats.

Treatment	Body weight (g)		Tissue weight (mg/100 g body weight)
	Initial	Final	Testes	Epididymis	Seminal vesicle	Ventral prostate	Vas deferens	Liver
Group I Control (vehicle treated)	180.00 ± 6.14	190.00 ± 7.96	1275.00 ± 42.53	402.00 ± 8.20	461.11 ± 9.12	271.20 ± 25.10	157.22 ± 13.33	3292.33 ± 27.13
Group II 0.2 mL oil/kg body weight for 60 days	186.62 ± 5.81	197.60 ± 8.42^ns	1064.76 ± 21.59*	397.44 ± 5.65*	418.89 ± 5.34*	218.70 ± 10.45*	142.89 ± 10.98*	3237.61 ± 22.96^ns
Group III 0.4 mL oil/kg body weight for 60 days	189.40 ± 6.43	198.76 ± 8.59^ns	902.67 ± 32.98**	353.27 ± 7.88**	387.09 ± 7.45**	184.32 ± 09.34**	139.56 ± 9.34**	3289.03 ± 30.90^ns
Group IV 0.5 mL oil/kg body weight for 60 days	190.00 ± 9.12	198.00 ± 6.16^ns	860.00 ± 69.20**	340.11 ± 3.66**	356.21 ± 3.66**	160.33 ± 16.33**	128.00 ± 10.10**	3267.11 ± 16.66^ns
Group V Recovery for 120 days after 60 days of treatment	185.00 ± 8.02	192.00 ± 9.62^ns	1185.00 ± 50.25^ns	390.00 ± 50.25^ns	436.66 ± 6.69^ns	256.80 ± 8.79^ns	152.38 ± 12.74^ns	3307.92 ± 22.24^ns

(Mean ± SEM of 10 animals). Group II, III, IV and V compared with Group I.

Ns, not significant; * P ≤ 0.01; **P ≤ 0.001.

Table 2.  Effect of Myristica fragrans oil on male rat fertility.

Treatment	No. of male	No. of female	No. of pregnant females	No. of implantation sites	No. of viable fetuses	Sperm motility (%) (cauda epididymis)	Sperm density (million/mL) (cauda epididymis)	Fertility test
Group I Control (vehicle treated)	10	20	20/20 (100%)	10.50 ± 03.05	09.02 ± 01.75	75.23 ± 00.91	52.22 ± 00.62	100% (-ve)
Group II 0.2 mL oil/kg bw for 60 days	10	20	9/20 (45%)	7.41 ± 02.67*	06.22 ± 03.48*	54.67 ± 0.61*	41.65 ± 00.89*	55% (-ve)
Group III 0.4 mL oil/kg bw for 60 days	10	20	3/20 (15%)	3.89 ± 01.65**	02.95 ± 02.22**	41.76 ± 00.38**	24.01 ± 00.45**	85% (-ve)
Group IV 0.5 mL oil/kg bw for 60 days	10	20	00/20 (0%)	Nil	Nil	25.66 ± 00.72**	13.33 ± 00.72**	100% (-ve)
Group V Recovery for 120 days after 60 days of treatment	10	20	16/20 (80%)	09.20 ± 04.44^ns	08.60 ± 02.02^ns	69.24 ± 01.20^ns	48.65 ± 01.39^ns	80% (+ve)

(Mean ± SEM of 10 animals). Group II, III, IV and V compared with Group I.

Ns, not significant; *P ≤ 0.01; **P ≤ 0.001.

Table 3.  Effect of Myristica fragrans oil on the level of serum testosterone.

Treatment		Serum testosterone (ng/mL)
Group I	Control (vehicle treated)	4.85 ± 0.97
Group II	0.2 mL oil/kg body weight for 60 days	3.53 ± 1.20*
Group III	0.4 mL oil/kg body weight for 60 days	2.22 ± 1.03**
Group IV	0.5 mL oil/kg body weight for 60 days	1.73 ± 0.33**
Group V	Recovery for 120 days after 60 days of treatment	4.37 ± 0.16^ns

(Mean ± SEM of 10 animals). Group II, III, IV and V compared with Group I.

ns, not significant; *P ≤ 0.01; **P ≤ 0.001.

Table 4.  Haematological changes of Myristica fragrans oil treated male rats.

Treatment	RBC counts (million/mm^3)	WBC counts (mm^3)	Hematocrit (%)	Hemoglobin (g%)	MCV μm^3	MCH Pg	MCHC %	Blood sugar (mg/100 mL)
Group I Control (vehicle treated)	5.02 ± 0.22	8817.77 ± 187.33	44.00 ± 0.82	13.96 ± 0.49	88.00 ± 0.20	27.92 ± 0.65	31.72 ± 0.95	90.10 ± 3.14
Group II 0.2 mL oil/kg bw for 60 days	5.18 ± 0.41^ns	8809.65 ± 115.42^ns	43.87 ± 0.90^ns	13.45 ± 0.74^ns	87.23 ± 0.67^ns	26.89 ± 0.50^ns	28.03 ± 0.63^ns	90.56 ± 2.76^ns
Group III 0.4 mL oil/kg bw for 60 days	5.72 ± 0.38^ns	8842.91 ± 184.01^ns	43.29 ± 0.73^ns	13.28 ± 0.88^ns	86.41 ± 0.42^ns	27.66 ± 0.10^ns	28.76 ± 0.80^ns	89.43 ± 3.87^ns
Group IV 0.5 mL oil/kg bw for 60 days	5.13 ± 0.33^ns	8802.00 ± 210.77^ns	43.66 ± 0.98^ns	12.21 ± 0.55^ns	85.60 ± 0.27^ns	23.94 ± 00.61^ns	27.96 ± 0.96^ns	91.43 ± 2.70^ns
Group V Recovery for 120 days after 60 days of treatment	5.60 ± 0.16^ns	8811.64 ± 192.66^ns	43.20 ± 0.86^ns	12.65 ± 0.69^ns	86.49 ± 0.18^ns	26.28 ± 0.63^ns	29.67 ± 0.84^ns	90.86 ± 2.94^ns

(Mean ± SEM of 10 animals). Group II, III, IV and V compared with Group I.

ns, not significant.

Figure 2.  Showing the section of a control Rat testis.

Figure 3.  Showing the section of Rat testis treated with Myristica fragrans Oil at the dose level of 0.2 ml/kg b. wt. for 60 days.

Figure 4.  Showing the section of Rat testis treated with Myristica fragrans Oil at the dose level of 0.4 ml/kg b. wt. for 60 days.

Figure 5.  Showing the section of Rat testis treated with Myristica fragrans Oil at the dose level of 0.5 ml/kg b. wt. for 60 days.

Figure 6.  Showing the section of Rat testis after the recovery period of 120 days.

Figure 7.  Effect of administration of Myristica fragrans Oil on the seminiferous tubule count of male albino rats.

Figure 8.  Effect of administration of Myristica fragrans Oil on the seminiferous tubule diameter of male albino rats.

Figure 9.  Effect of administration of Myristica fragrans Oil on the Leydig cell area of male albino rats.

Figure 10.  Effect of administration of Myristica fragrans Oil on the Leydig cell count of male albino rats.