Isolation, structural elucidation of flavonoid constituents from Leptadenia pyrotechnica and evaluation of their toxicity and antitumor activity

Figures & data

Figure 1.  Thin-layer chromatography of column chromatography fractions. (A) Detection under ultra-violet light. (B) Detection after spraying with AlCl₃ reagent.

Figure 2.  HPLC of Component E-I.1 and E-I.1a. (A) Analytical HPLC. (B) Preparative HPLC.

Figure 3.  Analytical HPLC of component E-I.2.

Figure 4.  Analytical HPLC of component E-II.2.

Figure 5.  HPLC of component E-III.1. (A) Analytical HPLC. (B) Preparative HPLC.

Figure 6.  ¹H-¹H-COSYT- experiment of component E-I.1.

Figure 7.  UV/Vis absorption spectra of compound E-I.2 in (A) MeOH and MeOH/NaOMe, (B) MeOH, MeOH/AlCl₃ and MeOH/AlCl₃/HCl and (C) MeOH, MeOH/NaOAc, and MeOH/NaOAc/H₃BO₃.

Table 1.  Mortality of brine shrimp at various concentration of the different extracts of Leptadenia pyrotechnica.

Plant extract	Dose ppm	Dosage log dose	Dead	Alive	Accumulated Dead	Accumulated Alive	Ratio Dead: Total	Mortality %	LD50 ppm
MeOH extract	1000	3	50	0	94	0	94/94	100	–
	100	2	44	6	44	6	44/50	88
	10	1	–	–	–	–	–	–
MeOH extract after defatting	1000	3	49	1	81	1	81/82	98.78	–
	100	2	32	18	32	19	32/51	62.74
	10	1	–	–	–	–	–	–
Dichloromethane extract	1000	3	50	0	50	0	50/50	100	–
	100	2	–	–	–	–	–	–
	10	1	–	–	–	–	–	–
EtOAc extract (Flavonoids)	1000	3	42	8	70	8	70/78	89.74	101.74
	100	2	23	27	28	35	28/63	44.44
	10	1	5	45	5	80	5/85	5.88
Flavonoid fraction E-I (11–13 )	1000	3	–	–	–	–	–	–	–
	100	2	16	34	16	34	16/50	32
	10	1	–	–	–	–	–	–
Flavonoid fraction E-II (14–17 )	1000	3	–	–	–	–	–	–	–
	100	2	15	35	15	35	15/50	30
	10	1	–	–	–	–	–	–
Flavonoid fraction E-III (18–19 )	1000	3	–	–	–	–	–	–	–
	100	2	30	20	30	20	30/50	60
	10	1	–	–	–	–	–	–
Flavonoid fraction E-I.1 ( 21–32 )	1000	3	–	–	–	–	–	–	–
	100	2	13	37	13	37	13/50	26
	10	1	–	–	–	–	–	–
Flavonoid fraction E-I.2 (33–38 )	1000	3	–	–	–	–	–	–	–
	100	2	46	4	46	4	46/50	92
	10	1	–	–	–	–	–	–
Flavonoid fraction E-II.1 ( 11–14 )	1000	3	–	–	–	–	–	–	–
	100	2	37	13	37	13	37/50	74
	10	1	–	–	–	–	–	–
Flavonoid fraction E-II.2 (15–16 )	1000	3	–	–	–	–	–	–	–
	100	2	37	13	37	13	37/50	74
	10	1	–	–	–	–	–	–
Flavonoid fraction E-IV (20–25)	1000	3	–	–	–	–	–	–	–
	100	2	33	17	33	17	33/50	66
	10	1	–	–	–	–	–	–