Moringa oleifera leaves as an inhibitor of human platelet aggregation

Figures & data

Figure 1.  Effect of aqueous extract of Moringa oleifera leaves on human platelet aggregation induced by collagen, ADP and epinephrine. *Percentage inhibition of aggregation relative to control.

Table 1.  IC₅₀ (mg)* of aqueous extracts of Moringa oleifera leaves on agonist-induced platelet aggregation.

Extract	Collagen	ADP	Epinephrine
Raw	0.48 ± 0.03	0.70 ± 0.04	ND
Heat-treated	0.87 ± 0.04	ND	ND

Values are mean ± SD. Duration of incubation was 1 min.

*The concentration required to inhibit platelet aggregation by 50%.

ND, not determined.

Figure 2.  Effect of aqueous extract of heat-treated (100°C, 15 min) Moringa oleifera leaves on human platelet aggregation induced by collagen, ADP, and epinephrine. *Percentage inhibition of aggregation relative to control.

Table 2.  Effect of incubation time on inhibition of platelet aggregation with aqueous extracts of Moringa oleifera leaves at IC₅₀.

	Inhibition (%)*
	1 min	3 min	5 min	10 min
Raw
Collagen	49.56 ± 2.24^c	58.73 ± 1.80^b	69.84 ± 2.50^a	70.63 ± 1.50^a
ADP	50.10 ± 1.70^d	67.72 ± 0.98^c	74.77 ± 2.80^b	83.78 ± 2.35^a
Epinephrine^↑	38.18 ± 1.10^b	100^a	ND	ND
Heat-treated
Collagen	49.86 ± 1.32 ^a	42.35 ± 1.60 ^b	ND	ND

Values are mean ± SD. Values bearing different superscripts a, b, c in the same row differ significantly (p ≤ 0.05) from each other.

* Percentage inhibition of platelet aggregation relative to control.

^↑Due to 0. 80 mg of extract.

ND, not determined

Table 3.  Effect of aqueous extracts of Moringa oleifera leaves on malonaldehayde (MA) formed in agonist challenged platelets at IC₅₀.

Sample	MA formed (nmol/1.6 × 10^7 platelets)
	Collagen	ADP	Epinephrine
Control*	556.25 ± 21.30^a	501.41 ± 22.47^a’	391.73 ± 17.50^a’’
Moringa oleifera
Raw	365.06 ± 13.45^c	352.55 ± 15.90^c’	274.21 ± 12.20^↑b’’
Heat-treated	413.53 ± 10.35^b	ND	ND

Values are mean ± SD. Values bearing different superscripts a, b, c in the same column differ significantly (p ≤ 0.05) from each other.

*Control represents platelets stimulated aggregation without addition of any inhibitor.

^↑Due to 0. 80 mg of extract.

ND, not determined.