Evaluation of the anti-inflammatory activity of N, N′-bis(3-dimethylamino-1-phenyl-propylidene)hydrazine dihydrochloride

Figures & data

Figure 1.  D1: N,N′-bis(3-Dimethylamino-1-phenyl-propylidene)hydrazine dihydrochloride.

Figure 2.  Changes in paw volume for 3 h period in D1 (100 mg kg⁻¹) and indomethacin (25 mg kg⁻¹) treated rats in comparison with control after injection of carrageenan.

Table 1.  The effects of D1, N,N′-bis(3-dimethylamino-1-phenyl-propylidene)hydrazine dihydrochloride, and indomethacin on carrageenan-induced rat paw edema.

Compound	No. of rats	Dose (mg kg^−1)	Paw volume (mL) before injection of carrageenan	Paw volume (mL) with inflammation after injection of carrageenan (anti-inflammatory activity)
				After 1 h	p	After 2 h	p	After 3 h	p
D1	6	50	0.90 ± 0.08	1.02 ± 0.2 (40%)	<0.05	1.29 ± 0.28 (22.5%)	>0.05	1.28 ± 0.36 (15.6%)	>0.05
D1	6	100	0.88 ± 0.08	1.04 ± 0.25 (20%)	<0.05	1.21 ± 0.24 (32.7%)	<0.05	1.18 ± 0.15 (33.4)	<0.05
D1	6	200	0.87 ± 0.06	1.05 ± 0.19 (10%)	>0.05	1.22 ± 0.17 (28.6%)	<0.05	1.28 ± 0.33 (8.9%)	>0.05
Indomethacin	6	25	0.89 ± 0.07	0.95 ± 0.11 (67.5%)	<0.05	0.95 ± 0.10 (87.8%)	<0.05	0.93 ± 0.12 (91.12%)	<0.05
Control	6	—	0.80 ± 0.06	1.0 ± 0.23	—	1.29 ± 0.31	—	1.25 ± 0.29	—

Note. Results are given as mean ± SD.

Table 2.  The effects of D1 and indomethacin on hyaluronidase-induced capillary permeability in rabbits.

Compound/drug	No. of rabbits	Dose (mg kg^−1)	Blue area spread of hyaluronidase (mm^2)
			After 1 min	p	After 5 min	p	After 30 min	p	After 60 min	p
D1	6	100	172.6 ± 41.6	>0.05	210.2 ± 39.7	>0.05	363 ± 50.2	>0.05	400.2 ± 46.7	>0.05
Indomethacin	6	25	38.8 ± 3.7	<0.03	48.2 ± 4.5	<0.001	100.6 ± 6.9	<0.001	119.8 ± 22.5	<0.002
Control	6	—	125.4 ± 18.5	—	243.4 ± 31.5	—	452.2 ± 83	—	496 ± 76.9	—

Note. Results are given as mean ± SD.