Figures & data
Figure 1. Effect of Standardized myrtol on inflammatory cell counts in BALF of LPS-induced ALI mice. Standardized myrtol at doses of 1200 mg/kg were administrated 1.5 h before LPS challenge. Mice were killed 6 h after LPS challenge and bronchoalveolar lavage was performed. The numbers of total cells (A) and neutrophils (B) in BALF were examined; (C) Mice were sacrificed 6 h after LPS challenge and lung tissues were homogenized in PBS. The homogenate was used for MPO assay. MPO activities were expressed as units per gram of protein. All values are mean ± SEM (n = 6). #p < 0.05, significant compared with vehicle-treated control; *p < 0.05, significant compared with LPS alone; **p < 0.01, significant compared with LPS alone.
Figure 2. Effect of standardized myrtol on total protein concentration in BALF of LPS-induced ALI mice. Standardized myrtol at doses of 1200 mg/kg were administrated 1.5 h before LPS challenge. Mice were killed 6 h after LPS challenge and bronchoalveolar lavage was performed. Total protein concentration in BALF was examined. All values are mean ± SEM (n = 6). #p < 0.05, significant compared with vehicle-treated control; *p < 0.05, significant compared with LPS alone; **p < 0.01, significant compared with LPS alone.
Figure 3. Effect of standardized myrtol on the levels of TNF-α and IL-6 in BALF of LPS-induced ALI mice. Standardized myrtol at doses of 1200 mg/kg were administrated 1.5 h before LPS challenge. Mice were killed 6 h after LPS challenge and bronchoalveolar lavage was performed. The levels of TNF-α (A) and IL-6 (B) in BALF were examined. All values are mean ± SEM (n = 6). #p < 0.05, significant compared with vehicle-treated control; *p < 0.05, significant compared with LPS alone; **p < 0.01, significant compared with LPS alone.
Figure 4. Standardized myrtol ameliorated LPS-induced histological changes in lung tissues. Representative lung sections are shown. 6 h after LPS challenge lungs were fixed, embedded in paraffin and cut into 5 μm slices. After H&E staining, histological examination was performed by light microscopy. Lung sections were obtained from vehicle-treated mice, Standardized myrtol (1200 mg/kg) treated mice, LPS-treated mice or LPS + Standardized myrtol (1200 mg/kg) treated mice (A), and lung injury was scored (B). All values are mean ± SEM (n = 6). #p < 0.05, significant compared with vehicle-treated control; *p < 0.05, significant compared with LPS alone; **p < 0.01, significant compared with LPS alone.
Figure 5. Standardized myrtol inhibited LPS-induced NF-κB activation in lung tissues. Standardized myrtol at doses of 1200 mg/kg were administrated 1.5 h before LPS challenge. Mice were killed 3 h after LPS inhalation and the lungs were removed. The extraction of nuclear and cytoplasmic proteins from lung tissues was performed. The expression of nuclear NF-κB p65 was detected by Western blotting. All values are mean ± SEM (n = 4). **p < 0.01, significant compared with LPS alone.
