Reproductive outcomes in patients with high levels of sperm DNA fragmentation using testicular sperm for intracytoplasmic injection: a retrospective analysis

Figures & data

Table 1. Treatment group characteristics of groups T-ICSI and E-ICSI.

	T-group	E-group	p value
No. of cycles/patients	37	36
Female age (years)	31.54 ± 4.69	31.05 ± 4.61	>0.05
Male age (years)	34.47 ± 5.32	33.18 ± 5.89	>0.05
Duration of infertility (years)	4.38 ± 3.51	3.86 ± 3.21	>0.05
BMI of women (kg/m^2)	20.40 ± 2.39	21.56 ± 2.88	>0.05
AMH	4.91 ± 2.26	4.10 ± 2.81	>0.05
Semen DFI (%)	60.58 ± 16.41	53.25 ± 18.15	>0.05
Testicular DFI (%)	13.37 ± 14.60	20.34 ± 19.64	>0.05
No of retrieved oocytes, n	14.86 ± 6.28	13.06 ± 5.20	>0.05
Mature oocytes (MII), n	12.32 ± 6.28	10.61 ± 4.81	>0.05
Sperm concentration (10^6/ml)	35.90(12.0,88.5)	13.95(3.8,73.7)	>0.05
Total sperm number (10^6)	68.74(26.2,243.1)	34.570(14.4,182.4)	>0.05
Sexual abstinence (days)	4.00(3.0,5.0)	4.000(3.3,5.0)	>0.05
Progressive motility (%)	9.60(1.8,22.3)	10.80(4.3,23.0)	>0.05

Table 2. Stimulation characteristics of groups T-ICSI and E-ICSI.

	T-group	E-group	p value
No. of cycles	37	36
Follicles (mean, range)	18.43 ± 7.59	17.65 ± 4.94	>0.05
Gonadotropin stimulation
Day of stimulation (mean)	10.44 ± 2.06	10.83 ± 1.80	>0.05
Total dose (mean)	1876.56 ± 393.22	1991.74 ± 713.48	>0.05
Endometrium (mean, range)	12.98 ± 2.28	12.35 ± 2.57	>0.05
Oestradiol (mean)	10392.06 ± 3825.52	8307.78 ± 4058.20	>0.05
Progesterone (mean)	2.78 ± 1.15	3.00 ± 1.42	>0.05

Table 3. Comparative embryologic and clinical outcomes between T-ICSI and E-ICSI.

	T-group	E-group	P value
Patients (n)	37	36
Maturation rate (MII/COC) (%)	82.91%(456/550)	81.28%(382/470)	>0.05
Fertilization rate (2PN/MII) (%)	72.15%(329/456)	77.23%(295/382)	>0.05
Embryo cleavage rate (d2/2PN) (%)	96.66%(318/329)	97.63%(288/295)	>0.05
High-quality embryo rate (%)	47.17%(150/318)	46.53%(134/288)	>0.05
Blastocyst formation rate (%)	33.85%(66/195)	21.56%(36/167)	<0.05*
Embryo transfer cycles (n)	16	23
Implantation rate (sacs/ET) (n, %)	40%(12/30)	34.78%(16/46)	>0.05
HCG positivity rate	62.50%(10/16)	60.87%(14/23)	>0.05
Clinical pregnancy rate (/ETC) (n, %)	50%(8/16)	56.52%(13/23)	>0.05
Singletons (/CP) (n, rate)	50%(4/8)	76.92%(10/13)	>0.05
Twins (/CP) (n, rate)	50%(4/8)	23.1%(3/13)	>0.05
Ectopic pregnancy (/CP) (n, rate)	0	0
Miscarriage (/CP) (n, rate)	12.5%(1/8)	23.08%(3/13)	>0.05
No. of patients have embryo suitable for freezing in each group	33	22
FET cycles	34(34/37)	28(28/36)	>0.05
Ongoing pregnancy (n, rate)	87.5%(7/8)	76.92%(10/13)	>0.05
Cumulative pregnancy rate (n, rate)	70.2%(26/37)	55.6%(20/36)	>0.05
Stillbirth (n, rate)	0	0
Live birth delivery rate (/ETC) (delivery-stillbirth) (n, rate)	43.75%(7/16)	43.48%(10/23)	>0.05

Table 4. Characteristics of the newborn between T-ICSI and E-ICSI.

	T-group	E-group	P value
Patients (n)	37	36
Embryo transfer cycles (ETC) (n)	16	23
Newborn (/ETC) (n, rate)	62.5%(10/16)	56.52%(13/23)	>0.05
Male (/NB) (n, rate)	30%(3/10)	30.77%(4/13)	>0.05
Female (/NB) (n, rate)	70%(7/10)	69.23%(9/13)	>0.05
M/F ratio	3/7	4/9
F/M ratio	7/3	9/4
NB malformations (/NB) (n, rate)	0	0
Gestation age (weeks) (n, mean, range)	37.99 ± 1.00	37.51 ± 2.68	>0.05
Term (n, mean, rate)	100%(10/10)	69.23%(9/13)	>0.05
Preterm (PT) (n, mean, rate)	0	30.77%(4/13)
Very PT (n, mean, rate)	0	0
Weight (g) (n, mean)	2677.00 ± 309.05	3007.69 ± 682.74	>0.05
Normal weight (n, mean, rate)	70%(7/10)	84.62%(11/13)	>0.05
Low weight (LW) (n, mean, rate)	30%(3/10)	15.38%(2/13)	>0.05
Very LbW (n, mean, rate)	0	0