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Expert Review of Molecular Diagnostics Volume 21, 2021 - Issue 7
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Review

Metabolomics-based predictive biomarkers of radiation injury and countermeasure efficacy: current status and future perspectives

Vijay K Singha Division of Radioprotectants,Department of Pharmacology and Molecular Therapeutics, F. Edward Hébert School of Medicine, Uniformed Serices University of the Health Sciences, Bethesda, MD, USA;b Scientific Research Department, Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD, USACorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-6631-3849View further author information
ORCID Icon,
Thomas M Seedc Tech Micro Services, Bethesda, MD, USAView further author information
&
Amrita K Cheemad Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA;e Department of Biochemistry, Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC, USAView further author information
Pages 641-654 | Received 20 Jan 2021, Accepted 19 May 2021, Published online: 07 Jun 2021

Figures & data

Figure 1. Flow diagram of non-targeted (global profiling) and targeted (select metabolites) metabolomics. Non-targeted approach has used NMR (Nuclear magnetic resonance), LC-MS (Liquid chromatography–mass spectrometry), and GC-MS (Gas chromatography–mass spectrometry) while targeted approach has used HPLC (High-performance liquid chromatography), UPLC (Ultra-performance liquid chromatography), LC-MS, and GC-FID (Gas chromatography – Flame-ionization detection)

Figure 1. Flow diagram of non-targeted (global profiling) and targeted (select metabolites) metabolomics. Non-targeted approach has used NMR (Nuclear magnetic resonance), LC-MS (Liquid chromatography–mass spectrometry), and GC-MS (Gas chromatography–mass spectrometry) while targeted approach has used HPLC (High-performance liquid chromatography), UPLC (Ultra-performance liquid chromatography), LC-MS, and GC-FID (Gas chromatography – Flame-ionization detection)

Figure 2. Experimental approach and analytical design of the metabolomic study using irradiated animals treated with radiation countermeasures. Blood/tissue samples are collected from irradiated or countermeasure-treated plus irradiated animals on various time-points pre and post-irradiation/treatment, and processed for untargeted LC-MS profiling. Metabolomics pathway and network analysis are analyzed, and validation is performed. LC-MS (Liquid chromatography–mass spectrometry), Tandem MS (Mass spectrometry)

Figure 2. Experimental approach and analytical design of the metabolomic study using irradiated animals treated with radiation countermeasures. Blood/tissue samples are collected from irradiated or countermeasure-treated plus irradiated animals on various time-points pre and post-irradiation/treatment, and processed for untargeted LC-MS profiling. Metabolomics pathway and network analysis are analyzed, and validation is performed. LC-MS (Liquid chromatography–mass spectrometry), Tandem MS (Mass spectrometry)

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