Template synthesis, spectroscopic studies and biological activities of macrocyclic complexes derived from thiocarbohydrazide and glyoxal

Figures & data

Table I.  Analytical data for the divalent cobalt, nickel, copper, zinc and cadmium complexes.

	Found(calcd) %
Sr. no.	Complexes	M	C	H	N	Colour	Mol. wt.
1.	[Co(TML)Cl2]	15.10 (15.28)	18.37 (18.65)	1.96 (2.07)	28.87 (29.01)	Light orange	386
2.	[Co(TML)(NO3)2]	13.21 (13.43)	16.27 (16.40)	1.76 (1.82)	31.66 (31.89)	Light orange	439
3.	[Co(TML)(OAc)2]	13.50 (13.62)	27.48 (27.71)	3.13 (3.23)	25.60 (25.86)	Brown	433
4.	[Ni(TML)Cl2]	14.98 (15.06)	18.56 (18.70)	1.99 (2.07)	29.01 (29.09)	Light pink	385
5.	[Ni(TML)(NO3)2]	13.14 (13.24)	16.34 (16.43)	1.57 (1.82)	31.32 (31.60)	Light grey	438
6.	[Ni(TML)(OAc)2]	13.34 (13.42)	27.70 (27.77)	3.08 (3.24)	25.67 (25.92)	Brown	432
7.	[Cu(TML)Cl2]	16.10 (16.15)	18.29 (18.46)	1.91 (2.05)	28.42 (28.71)	Yellow	390
8.	[Cu(TML)(NO3)2]	14.09 (14.22)	16.13 (16.25)	1.69 (1.80)	31.43 (31.60)	Brown	443
9	[Cu(TML)(OAc)2]	14.36 (14.41)	27.39 (27.45)	3.14 (3.20)	25.50 (25.62)	Dark green	437
10	[Zn(TML)(OAc)2]	-	27.19 (27.33)	3.10 (3.18)	25.34 (25.51)	Brown	439
11	[Cd(TML)(OAc)2]	-	24.21 (24.69)	2.59 (2.88)	22.88 (23.04)	Brown	486

Figure 1 Structure of the complexes.

Table II.  In vitro antibacterial spectrum of synthesized compounds using the agar diffusion assay.

		Diameter of zone of growth inhibition (mm)^a
Sr. no.	Compounds	Sa	Se	Pa	St
1	[Co(TML)Cl2]	15	–	–	10 ± .57
2	[Ni(TML)Cl2]	–	–	–	10 ± .57
3	[Cu(TML)Cl2]	10.83 ± .37	10 ± .81	10.83 ± .37	10.83 ± .37
4	[Cu(TML)(NO3)2]	15	15	10 ± .57	15
5	[Ni(TML)(NO3)2]	10.83 ± .37	–	–	10 ± .57
6	[Cd(TML)(OAc)2]	10 ± .81	10.83 ± .37	20	10.83 ± .37
7	[Zn(TML)(OAc)2]	15	10 ± .81	–	10.83 ± .37
8	[Co(TML)(NO3)2]	10 ± .81	10.83 ± .37	–	30
	DMSO	8	7.83	7.16	7.83

Sa- Staphylococcus aureus (MTCC 3160), Se- Staphylococcus epidermidis (MTCC 2639), Pa- Pseudomonas aeruginosa (MTCC 3541) and St-salmonella typhi (MTCC 733)

^a Mean of six replicates ± Standard deviation. –No activity.

Table III.  Minimum inhibitory concentration (MIC) of compounds against test bacteria using the agar dilution assay.

		MIC (μg/ mL)
Sr. no.	Compounds	Sa	Se	Pa	St
1	[Co(TML)Cl2]	32	–	–	>64
2	[Ni(TML)Cl2]	–	–	–	>64
3	[Cu(TML)Cl2]	64	>64	>64	>64
4	[Cu(TML)(NO3)2]	32	32	>64	16
5	[Ni(TML)(NO3)2]	>64	–	–	>64
6	[Cd(TML)(OAc)2]	>64	>64	16	>64
7	[Zn(TML)(OAc)2]	32	>64	–	>64
8	[Co(TML)(NO3)2]	>64	>64	–	4
	Cefuroxime axetial	32	16	32	16
	Linezolid	2	8	16	32

Sa- Staphylococcus aureus (MTCC 3160), Se- Staphylococcus epidermidis (MTCC 2639), Pa- Pseudomonas aeruginosa (MTCC 3541) and St- salmonella typhi (MTCC 733) Cefuroxime axetial and linezolid are standard antibiotics.

Figure 2 Comparison of minimum inhibitory concentration (MIC) of compounds and standard antibiotics against test microorganisms.