The inhibition kinetics of yeast glutathione reductase by some metal ions

Figures & data

Figure 1 Lineweaver-Burk double reciprocal plot of initial velocity against GSSG as varied substrate and ZnSO₄ (0.05–1 mM) as inhibitor at a fixed NADPH (0.1 mM) concentration. *0.1 mM NADPH (constant); □ 0.05 mM ZnSO₄ ;○ 0.1 mM ZnSO₄; Δ 0.5 mM ZnSO₄; ⋄1 mM ZnSO₄.

Figure 2 Lineweaver-Burk double reciprocal plot of initial velocity against NADPH as varied substrate and ZnSO₄ (0.05–1 mM) as inhibitor at different fixed GSSG (0.7 mM) concentrations. ⋄ 0.7 mM GSSG (constant); □ 0.05 mM ZnSO_4;Δ 0.1 mM ZnSO_4;*0.5 mM ZnSO_4;○ 1 mM ZnSO₄.

Figure 3 Lineweaver-Burk double reciprocal plot of initial velocity against GSSG as varied substrate and CaCl₂ (0.8–1.6 mM) as inhibitor at a fixed NADPH (0.1 mM) concentrations. *0.1 mM NADPH (constant); □ 0.8 mM CaCl₂; ○ 1 mm CaCl₂; Δ 1.2 mM CaCl₂; ⋄1.6 mM CaCl₂.

Figure 4 Lineweaver-Burk double reciprocal plot of initial velocity against NADPH as varied substrate and CaCl₂ (0.8–1.6 mM) as inhibitor at different fixed GSSG (0.7 mM) concentrations. ⋄ 0.7 mM GSSG (constant); □ 0.8 mM CaCl₂; Δ 1 mm CaCl₂; *1.2 mM CaCl₂; ○ 1.6 mM CaCl₂.

Figure 5 Lineweaver-Burk double reciprocal plot of initial velocity against GSSG as varied substrate and NiSO₄ (0.1–0.4 mM) as inhibitor at different fixed NADPH (0.1 mM) concentrations. *0.1 mM NADPH (constant); ○ 0.1 mM NiSO₄; ▪ 0.2 mM NiSO₄; Δ 0.3 mM NiSO₄; ⋄ 0.4 mM NiSO₄.

Figure 6 Lineweaver-Burk double reciprocal plot of initial velocity against NADPH as varied substrate and NiSO₄ (0.1–0.4 mM) as inhibitor at different fixed GSSG (0.7 mM) concentrations. ⋄ 0.7 mM GSSG (constant); □ 0.1 mM NiSO₄; Δ 0.2 mM NiSO₄; *0.3 mM NiSO₄; ○ 0.4 mM NiSO₄.