New antifungal flavone glycoside from Butea monosperma O. Kuntze

Figures & data

Table I.  ¹H-NMR (300 MHz, CDCl₃) spectrum of compound 1.

No.	δ Value	Pattern	J value (Hz)	No. of protons	Assignment
1.	6.71	d	8.3	1	H-8
2.	7.78	d	8.6	2	H-2′, H-6′
3.	7.21	d	8.5	2	H-3′, H-5′
4.	3.85	s		3	OMe-3
5.	3.93	s		3	OMe-6
6.	3.75	s		3	OMe-4′
7.	5.48	d	7.8	1	H-1″
8.	4.60	dd	3.6, 9.5	1	H-2″
9.	4.53	dd	9.6, 3.21	1	H-3″
10.	4.62	d	3.0	3	H-4″
11.	4.58	m		3	H-5″
12.	4.38	d	6.4	2	H-6″
13.	4.98	d	6.8	1	H-1″
14.	4.40	m		1	H-2″
15.	3.98	m		1	H-3″
16.	4.16	m		1	H-4″
17.	3.50	m		2	H-5″
18.	5.63	d	6.2	1	H-1″
19.	4.19	m		1	H-2″
20.	4.17	m		1	H-3″
21.	4.15	m		1	H-4″
22.	4.50	dd	10.6, 3.4	1	H-5″

Table II.  ¹³c-nmr (90 Mhz, Dmso-d₆) Spectrum Of Compound 1.

No.	δ Value	Assignment
1.	158.4	C-2
2.	135.8	C-3
3.	178.4	C-4
4.	162.5	C-5
5.	98.4	C-6
6.	167.2	C-7
7.	95.2	C-8
8.	157.8	C-9
9.	105.3	C-10
10	122.9	C-1′
11.	118.5	C-2′, C-6′
12.	115.8	C-3′, C-5′
13.	146.5	C-4′
14.	101.5	C-1″
15.	71.3	C-2″
16.	72.5	C-3″
17.	70.8	C-4″
18.	73.4	C-5″
19.	63.2	C-6″
20.	103.8	C-1″
21.	73.5	C-2″
22.	74.4	C-3″
23.	69.8	C-4″
24.	67.5	C-5″
25.	104.5	C-1″
26.	75.5	C-2″
27.	78.4	C-3″
28.	70.3	C-4″
29.	67.4	C-5″

Table III.  Antifungal activity of compound 1.

		Inhibition (mm)*
		Compound 1, at concentration (%)					Std**
No.	Fungal Species	100	80	60	40	20	100
1	Aspergillus niger	11.2	7.5	5.6	3.4	2.2	16.9
2.	Fusarium oxysporum	18.4	16.3	13.2	11.7	9.1	21.9
3.	Tricoderma viride	12.3	8.5	5.2	2.5	1.6	23.1
4.	Penicillium digitatum	17.5	15.4	13.4	9.5	6.4	23.5

*The zone of inhibition (mm) taken in different directions

**Griseofulvin used as standard antifungal agent.