Effects of some drugs on human erythrocyte 6-phosphogluconate dehydrogenase: an in vitro study

Figures & data

Figure 1 SDS-PAGE bands of 6PGD (Lane 1and 2: Human erythrocytes 6PGD. Lane 3: Ammonium sulfate precipitation. Lane 4: Hemolysate. Lane 5: Standards: Rabbit myosin (205,000), E. coli β-galactosidase (116,000), rabbit phosphorylase B (97,400), bovine albumin (66,000), chicken ovalbumin (45,000), and bovine carbonic anhydrase (29,000).

Figure 2 Activity % vs [Larnoxicam] regression analysis graphs for human erythrocytes 6PGD in the presence of 5 different larnoxicam concentrations.

Figure 3 Lineweaver-Burk graph with 5 different substrate (6PG) concentrations and 3 different larnoxicam concentrations for determination of K_i.

Table I.  Purification scheme for 6-phosphogluconate dehydrogenase from human erythrocytes.

Purification step	Total volume (mL)	Activity (U/mL)	Total activity (U)	Protein (mg/mL)	Specific activity (U/mg)	Yield (%)	Purification factor
Hemolysate	270	0.12	32.54	30.5	0.004	100	1
Ammonnium sulfate precipitation (35–65%)	75	0.40	30.14	35.6	0.011	92.22	2.80
2′,5′-ADP Sepharose 4B affinity chromatography	15	1.70	25.5	0.07	25.75	78	6552