Antibacterial and antifungal activities of andrachne cordifolia muell

Figures & data

Table I.  Antibacterial Activities Of Crude Extracts and Various fractions of Andrachne Cardifolia Muell.

Name of Bacteria	Zone of Inhibition of standard (Imipinem) 10 μg/mL	Crude Extract		CHCl3 Fraction		CH3COOEt Fraction		BuOH Fraction
		Zone of Inhibition (mm)	Inhibition (%)	Zone of Inhibition (mm)	Inhibition (%)	Zone of Inhibition (mm)	Inhibition (%)	Zone of Inhibition (mm)	Inhibition (%)
E.coli,	30	13.00	43%	Nil	Nil	Nil	Nil	17	53%
B. subitilis	33	15.00	46%	Nil	Nil	Nil	Nil	Nil	Nil
Sp aurous	33	Nil	Nil	Nil	Nil	Nil	Nil	Nil	Nil
Shigella flexenari	24	Nil	Nil	Nil	Nil	Nil	Nil	Nil	Nil
P. aerugenosa	24	11	46%	15	63%	15	63%	Nil	Nil
S. typhi	25	17	68%	25	100%	25	100%	7	28%

The plates were inculated at a concentration of 3 mg/mL of DMSO

Figure 1 Antibacterial activity towards P. aerugenosa and S. typhi of crude extract and subsequent fractions of Andrachne Cardifolia Muell. The extracts were used at a concentration of 3 mg/mL of DMSO.

Table II.  Antifungal activities of crude extracts and various fractions of Andrachne cardifolia Muell.

		Crude Extract		CHCl3 Fraction		CH3COOEt Fraction		BuOH Fraction		Standard Drugs
Name of fungi	Control Linear Growth (mm)	Linear Growth (mm)	Inhibition (%)	Linear Growth (mm)	Inhibition (%)	Linear Growth (mm)	Inhibition (%)	Linear Growth (mm)	Inhibition (%)	Name	MIC μl/mL
T. longi fusus	100	44	56%	30	50%	25	50%	70	30%	Miconazole	65
C. albicans	100	100	0%	100	0%	65	0%	80	20%	Miconazole	110.8
A. flavus	100	100	0%	35	0%	30	0%	100	0%	Amphotericin - B	24
M. canis	100	35	65%	30	76%	25	40%	75	25%	Miconazole	97
F. solani	100	50	50%	100	0%	100	0%	100	0%	Miconazole	85
C. glaberata	100	100	0%	100	0%	100	0%	100	0%	Miconazole	100.8

The concentration of relavant extract used was 24 mg/mL of DMSO.

Figure 2 Antifungal activity towards T. longifusus and M. cansis of crude extract and subseqent fractions of Andrachne cardifolia Muell. Final concentration of relavant extract was 24 mg/mL of DMSO.