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Research Article

Advances in assays of matrix metalloproteinases (MMPs) and their inhibitors

REVIEW

, &
Pages 154-167 | Received 24 Mar 2007, Accepted 12 May 2007, Published online: 04 Oct 2008

Figures & data

Figure 1 Schematic representation of the gelatinase assay using biotinylated gelatin as substrate.

Figure 1 Schematic representation of the gelatinase assay using biotinylated gelatin as substrate.

Figure 2 Schematic representation of the gelatinase assay using succinylated gelatin as substrate.

Figure 2 Schematic representation of the gelatinase assay using succinylated gelatin as substrate.

Figure 3 Schematic representation of the setup of the MMP-9 immunocapture activity assay.

Figure 3 Schematic representation of the setup of the MMP-9 immunocapture activity assay.

Figure 4 Schematic representation of western blotting and detection procedures.

Figure 4 Schematic representation of western blotting and detection procedures.

Table I.  The sensitivity and linearity of sandwich enzyme immunoassay for various MMP subtypes.

Table II.  The fluorophore, quencher, cleavage site and enzyme of various substrates*.

Figure 5 Structures of fluorogenic triple-helical peptide (fTHP) The top one using a covalent branching protocol and the bottom one using a peptide self-assembly approach.

Figure 5 Structures of fluorogenic triple-helical peptide (fTHP) The top one using a covalent branching protocol and the bottom one using a peptide self-assembly approach.

Figure 6 Outline of the tether phage selection.

Figure 6 Outline of the tether phage selection.

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