Figures & data
Scheme 1. The synthetic route of the linear polymer of cyclen copper (II) complex.
Figure 1. Effect of pH on the cleavage reaction of pUC 19 DNA (7 μg/mL) with complex 4 (1 mg/mL) in a Tris-HCl buffer (100 mM) at 37°C for 72 h. (A) Agarose gel electrophoresis diagram: lane 1, DNA control, pH 7.4; lane 2, pH 7.4; lane 3, DNA control, pH 7.0; lane 4, pH 7.0; lane 5, DNA control, pH 6.6; lane 6, pH 6.6; lane 7, DNA control, pH 6.2; lane 8, pH 6.2; lane 9, DNA control, pH 5.8; lane 10, pH 5.8. (B) quantitation of % plasmid relaxation relative to plasmid DNA per lane.
Figure 2. Effect of time on the cleavage reaction of pUC 19 DNA (7 μg/mL) with complex 4 (1 mg/mL) in a Tris-HCl buffer (100 mM, pH 7.4) at 37°C. (A) Agarose gel electrophoresis diagram: lane 1, DNA control, 0 h; lane 2, DNA control, 72 h; lane 3, 4 h; lane 4, 8 h; lane 5, 24 h; lane 6, 72 h. (B) quantitation of % plasmid relaxation relative to plasmid DNA per lane.
Figure 3. Effect of concentration of complex 4 on the cleavage reaction of pUC 19 DNA (7 μg/mL) with complex 4 in a Tris-HCl buffer (100 mM, pH 7.4) at 37°C. (A) Agarose gel electrophoresis diagram: lane 1, DNA control, 0 h; lane 2, DNA control, 72 h; lane 3, 0.2 mg/mL; lane 4, 0.4 mg/mL; lane 5, 0.6 mg/mL; lane 6, 0.8 mg/mL; lane 7, 1.0 mg/mL. (B) quantitation of % plasmid relaxation relative to plasmid DNA per lane.
