Figures & data
Figure 1 The effect of PN (peroxynitrite) and DPN (decomposed peroxynitrite) on CAT activity. (A) 10 μmol/L PN and DPN; (B) 20 μmol/L PN and DPN; (C) 40 μmol/L PN and DPN; (D) 50 μmol/L PN and DPN; (E) 100 μmol/L PN and DPN. Data are presented as absorbance at 240 nm versus time (s).
Figure 2 The effect of PN at various concentrations on CAT activity. Data are presented as percent absorbance change with respect to that of blank CAT activity, which was not incubated with PN.
Figure 3 The effect of DPN at various concentrations on CAT activity. Data are presented as percent absorbance change with respect to that of blank CAT activity, which was not incubated with DPN. DPN was obtained by preincubating PN solutions with the amounts of HCl equivalent to the basicity of PN solutions.
Figure 4 Percent inhibition of catalase activity with respect to increasing PN and DPN concentration based on measurements at 300 s. IC50 values were 5.5 and 8.5 μM for PN and DPN, respectively.
